Document Detail


Development of retinol-binding protein 4 immunocolloidal gold fast test strip using high-sensitivity monoclonal antibodies generated by DNA immunization.
MedLine Citation:
PMID:  21062789     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
DNA immunization is an efficient method for high-affinity monoclonal antibody generation. Here, we describe the generation of several high-quality monoclonal antibodies (mAbs) against retinol-binding protein 4 (RBP4), an important marker for kidney abnormality and dysfunction, with a combination method of DNA priming and protein boost. The mAbs generated could bind to RBP4 with high sensitivity and using these mAbs, an immunocolloidal gold fast test strip was constructed. The strip can give a result in <5 min and is very sensitive with a detection limit of about 1 ng/ml. A small-scale clinical test revealed that the result of this strip was well in accordance with that of an enzyme-labeled immunosorbent assay kit currently available on the market. Consequently, it could be useful for more convenient and faster RBP4 determination in the clinic.
Authors:
Chao Bian; Fang Zhang; Feng Wang; Zhiyang Ling; Min Luo; Hongqiang Wu; Yizhuo Sun; Junhui Li; Bingnan Li; Jingyan Zhu; Linna Tang; Yanyan Zhou; Qunfang Shi; Yongyong Ji; Lin Tian; Guomei Lin; Yu Fan; Niansong Wang; Bing Sun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-09
Journal Detail:
Title:  Acta biochimica et biophysica Sinica     Volume:  42     ISSN:  1745-7270     ISO Abbreviation:  Acta Biochim. Biophys. Sin. (Shanghai)     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101206716     Medline TA:  Acta Biochim Biophys Sin (Shanghai)     Country:  China    
Other Details:
Languages:  eng     Pagination:  847-53     Citation Subset:  IM    
Affiliation:
Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China. cbian@sibs.ac.cn
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