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Development of retinal pigment epithelium from human parthenogenetic embryonic stem cells and microRNA signature.
MedLine Citation:
PMID:  22736612     Owner:  NLM     Status:  Publisher    
PURPOSE. To investigate the potential of human parthenogenetic embryonic stem cells (hPESCs) to differentiate into retinal pigment epithelium (RPE) cells, and identify development-regulating microRNAs (miRNAs).METHODS. RPE cells were derived from hPESCs. The expression of markers and miRNA expression profiles during differentiation were studied by immunocytochemistry, real-time reverse transcription PCR (RT-PCR), and miRNA expression array at three time points. Human fetal RPE (hfRPE) cells were also analyzed. The target genes of candidate miRNAs were then validated. RESULTS. hPESC-derived RPE cells exhibited similar morphology and pigmentation to hfRPE cells. The expression of markers during differentiation indicated that the hPESC-derived RPE cells were immature. Most specific miRNAs played a role at some time point during the differentiation and maturation of RPE from hPESCs, except for two miRNAs (miR-204 and the miR-302 family). miR-204 was up-regulated and miR-302 was down-regulated throughout the process. Subsequently, pigmented clusters and RPE signature gene expression significantly increased in miR-204 overexpression group and miR-302 inhibition group compared with the control groups. CTNNBIP1 and TGFBR2 were confirmed to be the target genes of miR-204 and miR-302, respectively. CONCLUSIONS. hPESCs can develop into RPE-like cells, and can thus be additional promising sources of RPE cells in cell therapy. miR-204, miR-302s, and their targets are involved in regulating directed differentiation during the full course, thereby contributing to the search for a new method of improving differentiation efficiency using miRNAs.
Wen-Bo Li; Yun-Shan Zhang; Zhen-Yu Lu; Li-Jie Dong; Fei E Wang; Rong Dong; Xiao-Rong Li
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-26
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  -     ISSN:  1552-5783     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Tianjin Medical University Eye Centre, Tianjin, 300384, China.
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