| Development of resistance in Cronobacter sakazakii ATCC 29544 to thermal and non-thermal processes after exposure to stressing environmental conditions. | |
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MedLine Citation:
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PMID: 22221523 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Aims; The objective was to study the response of C. sakazakii ATCC 29544 cells to heat, pulsed electric fields (PEF), ultrasound under pressure (Manosonication, MS) and ultraviolet light (UV-C) treatments after exposure to different sublethal stresses that may be encountered in food-processing environments. Methods and Results: C. sakazakii stationary growth-phase cells (30 °C, 24 h) were exposed to acid (pH 4.5, 1 h), alkaline (pH 9.0, 1 h), osmotic (5% NaCl, 1 h), oxidative (0.5 mmol l(-1) H(2) O(2) , 1 h), heat (47.5 °C, 1 h), and cold (4 °C, 4 h) stress conditions and subjected to the subsequent challenges: heat (60 °C), PEF (25 kV cm(-1) , 35 °C), MS (117 μm, 200 kPa, 35 °C), and UV-C light (88.55 mW cm(-2) , 25 °C) treatments. The inactivation kinetics of C. sakazakii by the different technologies did not change after exposure to any of the stresses. The combinations of sublethal stress and lethal treatment that were protective were: heat shock-heat, heat shock-PEF, and acid pH-PEF. Conversely, the alkaline shock sensitized the cells to heat and UV-C treatments, the osmotic shock to heat treatments, and the oxidative shock to UV-C treatments. The maximum adaptive response was observed when heat-shocked cells were subjected to a heat treatment, increasing the time to inactivate 99.9% of the population by 1.6 times. Conclusions: C. sakazakii resistance to thermal and non-thermal preservation technologies can increase or decrease as a consequence of previous exposure to stressing conditions. Significance and Impact of the Study: The results help in understanding the physiology of the resistance of this emerging pathogen to traditional and novel preservation technologies. |
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Authors:
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C Arroyo; G Cebrián; S Condón; R Pagán |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-19 |
Journal Detail:
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Title: Journal of applied microbiology Volume: - ISSN: 1365-2672 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2012-1-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 The Society for Applied Microbiology. |
Affiliation:
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Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, University of Zaragoza, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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