Document Detail


Development of a repair-enrichment broth for resuscitation of heat-injured Listeria monocytogenes and Listeria innocua.
MedLine Citation:
PMID:  1531746     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of the divalent cations magnesium, iron, calcium and manganese; yeast extract; pyruvate; catalase; and the carbohydrates glucose, lactose, sucrose, esculin, fructose, galactose, maltose, and mannose to facilitate repair of heat-injured Listeria monocytogenes and Listeria innocua was evaluated. Listeria populations were injured by heating at 56 degrees C for 50 min. To determine the effects on repair, Trypticase soy broth (TSB) was supplemented with each medium component to be evaluated. Repair occurred to various degrees within 5 h in TSB supplemented with glucose, lactose, sucrose, yeast extract, pyruvate, or catalase. Chelex-exchanged TSB was supplemented with divalent cations; magnesium and iron cations were found to have a role in repair. Listeria repair broth (LRB) was formulated by utilizing the components that had the greatest impact upon repair. When incubated in LRB, heat-injured Listeria cells completed repair in 5 h. After the repair, acriflavin, nalidixic acid, and cycloheximide were added to LRB to yield final concentrations identical to those of the selective enrichment broths used in the procedures of the Food and Drug Administration and the U.S. Department of Agriculture. The efficacy of LRB in promoting repair and enrichment of heat-injured Listeria cells was compared with that of existing selective enrichment broths. Repair was not observed in the Food and Drug Administration enrichment broth, Listeria enrichment broth, or University of Vermont enrichment broth. The final Listeria populations after 24 h of incubation in selective enrichment media were 1.7 x 10(8) to 9.1 x 10(8) CFU/ml; populations in LRB consistently averaged 2.5 x 10(11) to 8.2 x 10(11) CFU/ml.
Authors:
S V Busch; C W Donnelly
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  58     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1992 Jan 
Date Detail:
Created Date:  1992-03-30     Completed Date:  1992-03-30     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  14-20     Citation Subset:  IM    
Affiliation:
Department of Animal Science, University of Vermont, Burlington 05405.
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MeSH Terms
Descriptor/Qualifier:
Calcium / metabolism
Carbohydrate Metabolism
Caseins*
Catalase / metabolism
Culture Media*
Hot Temperature
Iron / metabolism
Kinetics
Listeria / growth & development*,  metabolism
Listeria monocytogenes / growth & development*,  metabolism
Magnesium / metabolism
Protein Hydrolysates
Pyruvates / metabolism
Chemical
Reg. No./Substance:
0/Caseins; 0/Culture Media; 0/Protein Hydrolysates; 0/Pyruvates; 0/trypticase-soy broth; 7439-89-6/Iron; 7439-95-4/Magnesium; 7440-70-2/Calcium; EC 1.11.1.6/Catalase
Comments/Corrections

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