Document Detail

Development of a recombinant baculovirus expressing an insect-selective neurotoxin: potential for pest control.
MedLine Citation:
PMID:  1367359     Owner:  NLM     Status:  MEDLINE    
Recombinant nuclear polyhedrosis viruses (NPVs) expressing insect-selective toxins, hormones, or enzymes could enhance their insecticidal properties. We have constructed a recombinant, polyhedrin-positive Autographa californica NPV (AcNPV) that is orally infectious and expresses an insect-selective toxin (AaIT), isolated from the scorpion Androctonus australis, under the control of the p10 promoter. Bioassays with the recombinant baculovirus on 2nd instar larvae of Heliothis virescens demonstrated a significant decrease in the time to kill (LT50 88.0 hours) compared to wild-type AcNPV (LT50 125 hours). Production of AaIT was confirmed by western blot analysis of larval hemolymph from infected H. virescens, and bioassays with larvae of Sarcophaga falculata.
B F McCutchen; P V Choudary; R Crenshaw; D Maddox; S G Kamita; N Palekar; S Volrath; E Fowler; B D Hammock; S Maeda
Related Documents :
8508799 - Efficient secretion of attacin from insect fat-body cells requires proper processing of...
22966759 - Capsid protein expression and adeno-associated virus like particles assembly in sacchar...
24694399 - Characterization of human papillomavirus 6b l1 virus-like particles isolated from silkw...
14658849 - Synergism between the nucleopolyhedroviruses of autographa californica and trichoplusia...
1085219 - The biology, behaviour, and ecology of mastomys natalensis in southern africa.
24469789 - Neutralizing antibodies can initiate genome release from human enterovirus 71.
19369339 - Genetic evidence for a connection between rous sarcoma virus gag nuclear trafficking an...
14505899 - Bovine herpes virus gd protein produced in plants using a recombinant tobacco mosaic vi...
20716159 - Peroxisome proliferator-activated receptor and amp-activated protein kinase agonists pr...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Bio/technology (Nature Publishing Company)     Volume:  9     ISSN:  0733-222X     ISO Abbreviation:  Biotechnology (N.Y.)     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-10-10     Completed Date:  1991-10-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8309273     Medline TA:  Biotechnology (N Y)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  848-52     Citation Subset:  B    
Department of Entomology, University of California, Davis 95616.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Baculoviridae / genetics*
Base Sequence
Cell Line
Larva / drug effects
Lepidoptera / drug effects*
Molecular Sequence Data
Oligonucleotide Probes
Pest Control / methods*
Recombinant Proteins / isolation & purification,  pharmacology
Recombination, Genetic*
Restriction Mapping
Scorpion Venoms / genetics*,  isolation & purification,  pharmacology
Reg. No./Substance:
0/Neurotoxins; 0/Oligonucleotide Probes; 0/Recombinant Proteins; 0/Scorpion Venoms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Generation of transgenic dairy cattle using 'in vitro' embryo production.
Next Document:  Secretion of active bovine somatotropin in Escherichia coli.