Document Detail


Development of a recombinant baculovirus expressing an insect-selective neurotoxin: potential for pest control.
MedLine Citation:
PMID:  1367359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recombinant nuclear polyhedrosis viruses (NPVs) expressing insect-selective toxins, hormones, or enzymes could enhance their insecticidal properties. We have constructed a recombinant, polyhedrin-positive Autographa californica NPV (AcNPV) that is orally infectious and expresses an insect-selective toxin (AaIT), isolated from the scorpion Androctonus australis, under the control of the p10 promoter. Bioassays with the recombinant baculovirus on 2nd instar larvae of Heliothis virescens demonstrated a significant decrease in the time to kill (LT50 88.0 hours) compared to wild-type AcNPV (LT50 125 hours). Production of AaIT was confirmed by western blot analysis of larval hemolymph from infected H. virescens, and bioassays with larvae of Sarcophaga falculata.
Authors:
B F McCutchen; P V Choudary; R Crenshaw; D Maddox; S G Kamita; N Palekar; S Volrath; E Fowler; B D Hammock; S Maeda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Bio/technology (Nature Publishing Company)     Volume:  9     ISSN:  0733-222X     ISO Abbreviation:  Biotechnology (N.Y.)     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-10-10     Completed Date:  1991-10-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8309273     Medline TA:  Biotechnology (N Y)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  848-52     Citation Subset:  B    
Affiliation:
Department of Entomology, University of California, Davis 95616.
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MeSH Terms
Descriptor/Qualifier:
Animals
Baculoviridae / genetics*
Base Sequence
Cell Line
Immunoblotting
Larva / drug effects
Lepidoptera / drug effects*
Molecular Sequence Data
Neurotoxins*
Oligonucleotide Probes
Pest Control / methods*
Recombinant Proteins / isolation & purification,  pharmacology
Recombination, Genetic*
Restriction Mapping
Scorpion Venoms / genetics*,  isolation & purification,  pharmacology
Transfection*
Chemical
Reg. No./Substance:
0/Neurotoxins; 0/Oligonucleotide Probes; 0/Recombinant Proteins; 0/Scorpion Venoms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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