| Development of a recombinant baculovirus expressing an insect-selective neurotoxin: potential for pest control. | |
MedLine Citation:
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PMID: 1367359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recombinant nuclear polyhedrosis viruses (NPVs) expressing insect-selective toxins, hormones, or enzymes could enhance their insecticidal properties. We have constructed a recombinant, polyhedrin-positive Autographa californica NPV (AcNPV) that is orally infectious and expresses an insect-selective toxin (AaIT), isolated from the scorpion Androctonus australis, under the control of the p10 promoter. Bioassays with the recombinant baculovirus on 2nd instar larvae of Heliothis virescens demonstrated a significant decrease in the time to kill (LT50 88.0 hours) compared to wild-type AcNPV (LT50 125 hours). Production of AaIT was confirmed by western blot analysis of larval hemolymph from infected H. virescens, and bioassays with larvae of Sarcophaga falculata. |
Authors:
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B F McCutchen; P V Choudary; R Crenshaw; D Maddox; S G Kamita; N Palekar; S Volrath; E Fowler; B D Hammock; S Maeda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Bio/technology (Nature Publishing Company) Volume: 9 ISSN: 0733-222X ISO Abbreviation: Biotechnology (N.Y.) Publication Date: 1991 Sep |
Date Detail:
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Created Date: 1991-10-10 Completed Date: 1991-10-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8309273 Medline TA: Biotechnology (N Y) Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 848-52 Citation Subset: B |
Affiliation:
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Department of Entomology, University of California, Davis 95616. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Baculoviridae / genetics* Base Sequence Cell Line Immunoblotting Larva / drug effects Lepidoptera / drug effects* Molecular Sequence Data Neurotoxins* Oligonucleotide Probes Pest Control / methods* Recombinant Proteins / isolation & purification, pharmacology Recombination, Genetic* Restriction Mapping Scorpion Venoms / genetics*, isolation & purification, pharmacology Transfection* |
| Chemical | |
Reg. No./Substance:
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0/Neurotoxins; 0/Oligonucleotide Probes; 0/Recombinant Proteins; 0/Scorpion Venoms |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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