| Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples. | |
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MedLine Citation:
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PMID: 20466534 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A reliable and cost-effective electrochemical method for the detection of deoxynivalenol (DON) in cereals and cereal-based food samples based on the use of a novel anti-DON Fab fragment is presented. The analytical system employed, Enzyme-Linked-Immunomagnetic-Electrochemical (ELIME) assay, is based on the use of immunomagnetic beads (IMBs) coupled with eight magnetized screen-printed electrodes (8-mScPEs) as electrochemical transducers. Using standard solutions of DON, a working range between 100 and 4500 ng/ml was obtained with an EC(50) of 380 ng/ml. The ELIME assay was employed to evaluate the cross-reactivity of the Fab fragment towards different trichothecenes revealing a good selectivity towards DON over other trichothecenes with the exception of 3-Ac-DON. The sensor was then applied to cereals and cereal-based food samples (wheat, breakfast cereal and baby-food) and a wide range of sample treatment procedures was tested. Within-laboratory precision (9-24% repeatability for breakfast cereals and 10-33% for baby-food) and recovery data (82-110% for breakfast cereals and 97-108% for baby-food) were calculated by analyzing blank breakfast cereals and baby-foods fortified with DON, demonstrating that the proposed method has the capability for use as a screening assay for DON in such products. |
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Authors:
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Daniela Romanazzo; Francesco Ricci; Giulia Volpe; Christopher T Elliott; Silvia Vesco; Katy Kroeger; Danila Moscone; Joerg Stroka; Hans Van Egmond; Markus Vehniäinen; Giuseppe Palleschi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-28 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 25 ISSN: 1873-4235 ISO Abbreviation: Biosens Bioelectron Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-06-23 Completed Date: 2010-10-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
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Languages: eng Pagination: 2615-21 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Università di Roma, Tor Vergata, Dipartimento di Scienze e Tecnologie Chimiche, Rome, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Immobilized Biosensing Techniques / instrumentation, methods* Cereals / chemistry Electrochemical Techniques Food Contamination / analysis* Humans Immunoassay / methods Immunoglobulin Fab Fragments Infant Infant Food / analysis Recombinant Proteins Trichothecenes / analysis*, immunology Triticum / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Immobilized; 0/Immunoglobulin Fab Fragments; 0/Recombinant Proteins; 0/Trichothecenes; 51481-10-8/deoxynivalenol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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