Document Detail


Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples.
MedLine Citation:
PMID:  20466534     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A reliable and cost-effective electrochemical method for the detection of deoxynivalenol (DON) in cereals and cereal-based food samples based on the use of a novel anti-DON Fab fragment is presented. The analytical system employed, Enzyme-Linked-Immunomagnetic-Electrochemical (ELIME) assay, is based on the use of immunomagnetic beads (IMBs) coupled with eight magnetized screen-printed electrodes (8-mScPEs) as electrochemical transducers. Using standard solutions of DON, a working range between 100 and 4500 ng/ml was obtained with an EC(50) of 380 ng/ml. The ELIME assay was employed to evaluate the cross-reactivity of the Fab fragment towards different trichothecenes revealing a good selectivity towards DON over other trichothecenes with the exception of 3-Ac-DON. The sensor was then applied to cereals and cereal-based food samples (wheat, breakfast cereal and baby-food) and a wide range of sample treatment procedures was tested. Within-laboratory precision (9-24% repeatability for breakfast cereals and 10-33% for baby-food) and recovery data (82-110% for breakfast cereals and 97-108% for baby-food) were calculated by analyzing blank breakfast cereals and baby-foods fortified with DON, demonstrating that the proposed method has the capability for use as a screening assay for DON in such products.
Authors:
Daniela Romanazzo; Francesco Ricci; Giulia Volpe; Christopher T Elliott; Silvia Vesco; Katy Kroeger; Danila Moscone; Joerg Stroka; Hans Van Egmond; Markus Vehniäinen; Giuseppe Palleschi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-28
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  25     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-06-23     Completed Date:  2010-10-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  2615-21     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Affiliation:
Università di Roma, Tor Vergata, Dipartimento di Scienze e Tecnologie Chimiche, Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Immobilized
Biosensing Techniques / instrumentation,  methods*
Cereals / chemistry
Electrochemical Techniques
Food Contamination / analysis*
Humans
Immunoassay / methods
Immunoglobulin Fab Fragments
Infant
Infant Food / analysis
Recombinant Proteins
Trichothecenes / analysis*,  immunology
Triticum / chemistry
Chemical
Reg. No./Substance:
0/Antibodies, Immobilized; 0/Immunoglobulin Fab Fragments; 0/Recombinant Proteins; 0/Trichothecenes; 51481-10-8/deoxynivalenol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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