| Development of real-time radioisotope imaging systems for plant nutrient uptake studies. | |
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MedLine Citation:
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PMID: 22527392 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Ionic nutrition is essential for plant development. Many techniques have been developed to image and (or) measure ionic movement in plants. Nevertheless, most of them are destructive and limit the analysis. Here, we present the development of radioisotope imaging techniques that overcome such restrictions and allow for real-time imaging of ionic movement. The first system, called macroimaging, was developed to visualize and measure ion uptake and translocation between organs at a whole-plant scale. Such a device is fully compatible with illumination of the sample. We also modified fluorescent microscopes to set up various solutions for ion uptake analysis at the microscopic level. Both systems allow numerical analysis of images and possess a wide dynamic range of detection because they are based on radioactivity. |
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Authors:
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Satomi Kanno; Masato Yamawaki; Hiroki Ishibashi; Natsuko I Kobayashi; Atsushi Hirose; Keitaro Tanoi; Laurent Nussaume; Tomoko M Nakanishi |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Philosophical transactions of the Royal Society of London. Series B, Biological sciences Volume: 367 ISSN: 1471-2970 ISO Abbreviation: Philos. Trans. R. Soc. Lond., B, Biol. Sci. Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-04-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7503623 Medline TA: Philos Trans R Soc Lond B Biol Sci Country: England |
Other Details:
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Languages: eng Pagination: 1501-8 Citation Subset: IM |
Affiliation:
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Graduate School of Agricultural and Life Sciences, University of Tokyo, , 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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