Document Detail


Development of real-time radioisotope imaging systems for plant nutrient uptake studies.
MedLine Citation:
PMID:  22527392     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ionic nutrition is essential for plant development. Many techniques have been developed to image and (or) measure ionic movement in plants. Nevertheless, most of them are destructive and limit the analysis. Here, we present the development of radioisotope imaging techniques that overcome such restrictions and allow for real-time imaging of ionic movement. The first system, called macroimaging, was developed to visualize and measure ion uptake and translocation between organs at a whole-plant scale. Such a device is fully compatible with illumination of the sample. We also modified fluorescent microscopes to set up various solutions for ion uptake analysis at the microscopic level. Both systems allow numerical analysis of images and possess a wide dynamic range of detection because they are based on radioactivity.
Authors:
Satomi Kanno; Masato Yamawaki; Hiroki Ishibashi; Natsuko I Kobayashi; Atsushi Hirose; Keitaro Tanoi; Laurent Nussaume; Tomoko M Nakanishi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Philosophical transactions of the Royal Society of London. Series B, Biological sciences     Volume:  367     ISSN:  1471-2970     ISO Abbreviation:  Philos. Trans. R. Soc. Lond., B, Biol. Sci.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-04-24     Completed Date:  2012-08-06     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  7503623     Medline TA:  Philos Trans R Soc Lond B Biol Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1501-8     Citation Subset:  IM    
Affiliation:
Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / metabolism*
Biological Transport
Culture Media / metabolism
Image Processing, Computer-Assisted / instrumentation,  methods*
Iron Compounds / metabolism
Isotope Labeling / instrumentation,  methods*
Microscopy, Fluorescence / instrumentation,  methods*
Oryza sativa / metabolism*
Phosphates / metabolism
Phosphorus Isotopes / metabolism
Water / metabolism
Chemical
Reg. No./Substance:
0/Culture Media; 0/Iron Compounds; 0/Phosphates; 0/Phosphorus Isotopes; 7732-18-5/Water
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