| Development of real time PCR assays for detection and quantification of transgene DNA of a Bacillus thuringiensis (Bt) corn hybrid in soil samples. | |
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MedLine Citation:
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PMID: 20044792 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Real time PCR assays were developed to detect and quantify the transgene DNA of a commercially released Bacillus thuringiensis (Bt) corn (Zea mays L.) hybrid (DKC42-23), which was derived from the event MON863 and also carried a neomycin phosphotransferase gene (the nptII gene). We applied the real time PCR assays to investigate the persistence of the transgene DNA in a field trial grown with DKC42-23 over 3 years, in combination with bacterial natural transformation. The results showed that under continuous cultivation of DKC42-23, its transgene DNA was detectable in the field plots all year around. Meanwhile, when soil DNA extracts from DKC42-23 plots were used as donor in bacterial natural transformation, successful recovery of kanamycin resistant (Km(R)) transformants indicated that the nptII gene carried by DKC42-23 could be taken up and integrated into naturally competent Pseudomonas stutzeri pMR7 cells, leading to the restoration of the antibiotic resistance of P. stutzeri pMR7. However, after the cultivation of a soybean line in the same plots for the subsequent growing season, the presence of transgene DNA of DKC42-23 was reduced to undetectable levels at the end of that growing season. Therefore, existing corn-soybean crop rotation practices reduce the availability of transgene DNA in soil and thus minimize the risks that might be attributable to horizontal gene transfer. The real time PCR assays are useful for investigating the persistence of transgene DNA derived from the MON863 event in soil environments. |
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Authors:
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Bin Zhu; Bao-Luo Ma; Robert E Blackshaw |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-01 |
Journal Detail:
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Title: Transgenic research Volume: 19 ISSN: 1573-9368 ISO Abbreviation: Transgenic Res. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-09 Completed Date: 2011-01-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9209120 Medline TA: Transgenic Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 765-74 Citation Subset: IM |
Affiliation:
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Environment Canada, 11 Innovation Boulevard, Saskatoon, SK, S7N 3H5, Canada. bin.zhu@ec.gc.ca |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus thuringiensis
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genetics* Computer Systems* Crops, Agricultural / genetics DNA, Recombinant / analysis* Kanamycin Kinase / genetics Kanamycin Resistance / genetics Plants, Genetically Modified / genetics* Polymerase Chain Reaction / methods* Pseudomonas stutzeri / genetics Soil / analysis* Soil Microbiology* Soybeans / genetics, metabolism Transformation, Bacterial Transgenes* Zea mays / genetics* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Recombinant; 0/Soil; EC 2.7.1.95/Kanamycin Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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