Document Detail


Development of a rare cell fractionation device: application for cancer detection.
MedLine Citation:
PMID:  15631136     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Isolating rare cells from biological fluids including whole blood or bone marrow is an interesting biological problem. Characterization of a few metastatic cells from cancer patients for further study is desirable for prognosis/diagnosis. Traditional methods have not proven adequate, due to the compositional complexity of blood, with its large numbers of cell types. To separate individual cells based on their mechanical characteristics, we have developed a series of massively parallel microfabricated sieving device. These devices were constructed with four successively narrower regions of channels numbering approximately 1800 per region. As cells traversed the device, they encountered each region and stopped at a gap width that prohibited passage due to their size. Cultured neuroblastoma cells, when mixed with whole blood and applied to the device, were retained in the 10-microm-wide by 20-microm-deep channels. All other cells migrated to the output. A derivative of the same device was utilized to characterize migration of whole blood. Adult white blood cells were retained at the 2.5-microm-wide by 5-microm-deep channels, while red blood cells passed through these channels. Devices designed to capture rare cells in peripheral circulation for downstream analysis will provide an important tool for diagnosis and treatment.
Authors:
Hisham Mohamed; Leslie D McCurdy; Donald H Szarowski; Salvatore Duva; James N Turner; Michele Caggana
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  IEEE transactions on nanobioscience     Volume:  3     ISSN:  1536-1241     ISO Abbreviation:  IEEE Trans Nanobioscience     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2005-01-05     Completed Date:  2005-03-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101152869     Medline TA:  IEEE Trans Nanobioscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  251-6     Citation Subset:  IM    
Affiliation:
Wadsworth Center, New York State Department of Health, Albany, NY 12201, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Separation / instrumentation*,  methods
Cytapheresis / instrumentation,  methods
Equipment Design
Equipment Failure Analysis
Erythrocytes / cytology*
Leukocytes / cytology*
Microfluidic Analytical Techniques / instrumentation*,  methods
Nanotechnology / instrumentation*,  methods
Neuroblastoma / blood,  pathology*
Ultrafiltration / instrumentation*,  methods

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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