Document Detail

Development of a rapid and sensitive enzyme-linked immunosorbent assay (ELISA) for measuring venom antigens after an experimental snake bite.
MedLine Citation:
PMID:  3238700     Owner:  NLM     Status:  MEDLINE    
We describe a new ELISA which allows the measurement of the concentration of venom antigens in whole blood. The assay can be performed in less than 20 min and requires a 200 microliters sample of blood. It allows the accurate evaluation of concentrations of Vipera ammodytes venom in quantities smaller than 1 ng/ml of blood. Using this ELISA, we were able to follow in rabbits the kinetics of experimental envenomation with non-lethal doses of venom. This ELISA was also used to measure post mortem the level of venom antigens in various tissues such as liver, kidney, muscles and abdominal serosity of a rabbit. The method, which might be adapted to measure envenomation by other snake species, seems to be sufficiently rapid and sensitive to allow routine evaluation of the gravity of a snake bite in humans and to estimate the efficacy of immunotherapy.
H Labrousse; A K Nishikawa; C Bon; S Avrameas
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  26     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  1988  
Date Detail:
Created Date:  1989-05-01     Completed Date:  1989-05-01     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1157-67     Citation Subset:  IM    
Unité d'Immunocytochimie, UA 359, CNRS, Institut Pasteur, Paris, France.
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MeSH Terms
Antigens / analysis*
Enzyme-Linked Immunosorbent Assay / methods
Immunization, Passive
Snake Bites / diagnosis*,  therapy
Snake Venoms / analysis,  immunology*
Reg. No./Substance:
0/Antigens; 0/Snake Venoms

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