| Development of a rapid method for the measurement of lactose in milk using a blood glucose biosensor. | |
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MedLine Citation:
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PMID: 21943731 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Current methods for lactose measurement in dairy products are time consuming and tedious and may require expensive equipment and skilled technicians. The aim of this research was to develop a novel and rapid method for the routine measurement of lactose in dairy products. The proposed method is based on the rapid hydrolysis of lactose using β-galactosidase and subsequently measuring glucose using a blood glucose meter. Blood glucose meters were developed after decades of research and clinical trials and are used extensively worldwide by individuals with diabetes to monitor their blood glucose levels. The method was developed and validated in a series of experiments. In the first experiment, temperature and time required for the near-complete hydrolysis of lactose were determined. Subsequently, the influence of glucose meters and their test strip lots were evaluated. We found that meters were not significantly different. However, the test strip lots were significantly different from each other. In the second experiment, the proposed method was validated using different concentrations of lactose solutions (1.9-6.5%) and compared with a HPLC-based reference method. In the third experiment, the proposed method was used to determine the lactose content of raw milk. The proposed method shows potential for rapid, routine, and low-cost measurement of lactose in milk and other dairy products. |
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Authors:
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J K Amamcharla; L E Metzger |
Publication Detail:
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Type: - |
Journal Detail:
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Title: Journal of dairy science Volume: 94 ISSN: 1525-3198 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-9-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: - |
Other Details:
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Languages: ENG Pagination: 4800-4809 Citation Subset: - |
Copyright Information:
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Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Midwest Dairy Foods Research Center, Dairy Science Department, South Dakota State University, Brookings 57007-0647. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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