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Development of a rapid low cost fluorescent biosensor for the detection of food contaminants.
MedLine Citation:
PMID:  22939510     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A prototype fluorescent based biosensor has been developed for the antibody based detection of food related contaminants. Its performance was characterised and showed a typical antibody binding signal of 200-2000mV, a short term noise of 9.1mV, and baseline slope of -0.016mV/s over 4h. Bulk signal detection repeatability (n=23) and reproducibility (n=3) were less than 2.4%CV. The biosensor detection unit was evaluated using two food related model systems proving its ability to monitor both binding using commercial products and inhibition through the development of an assay. This assay development potential was evaluated by observing the biosensor's performance whilst appraising several labelled antibody and glass slide configurations. The molecular interaction between biotin and an anti-biotin antibody was shown to be inhibited by 41% due to the presence of biotin in a sample. A food toxin (domoic acid) calibration curve was produced, with %CVs ranging from 2.7 to 7.8%, and a midpoint of approximately 17ng/ml with further optimisation possible. The ultimate aim of this study was to demonstrate the working principles of this innovative biosensor as a potential portable tool with the opportunity of interchangeable assays. The biosensor design is applicable for the requirements of routine food contaminant analysis, with respect to performance, functionality and cost.
Authors:
Terry F McGrath; Karl Andersson; Katrina Campbell; Terry L Fodey; Christopher T Elliott
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-16
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  -     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-9-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
ASSET Technology Centre, Institute of Agri-Food and Land Use, School of Biological Sciences, Queen's University, Stranmillis Road, Belfast BT9 5AG, Northern Ireland.
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