| Development of a rapid enzyme immunoassay for the detection of retinol-binding protein. | |
| | |
MedLine Citation:
|
PMID: 14684403 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Retinol-binding protein (RBP) was chosen as a surrogate marker for retinol because of the close correspondence between retinol and RBP. OBJECTIVE: To meet the need for rapid, cost-effective determination of vitamin A status in populations, a quantitative enzyme immunoassay (EIA) for detection of RBP was developed. DESIGN: The resulting RBP EIA, a competitive assay, uses RBP adsorbed to microtest strip wells to compete with RBP in serum. The assay takes approximately 40 min. RESULTS: With a reference panel of sera, test accuracy was found to be within 4% of expected values through the calibrated range of 0.48-1.92 micro mol RBP/L (10-40 micro g RBP/mL). Intraassay and interassay variability averaged 6.7% and 8.9%, respectively. Specificity testing showed no interference from other serum proteins, prealbumin, rheumatoid factor, bilirubin, estrogen, or C-reactive protein. The RBP EIA provided linear results between 0.43 and 1.80 micro mol RBP/L (9 and 38 micro g RBP/mL). Preliminary laboratory evaluations indicated that the RBP EIA correlates well with radial immunodiffusion for RBP and with HPLC for retinol, the current reference standard. A field evaluation in a population at risk for vitamin A deficiency (VAD) resulted in close correlation between RBP EIA measures and retinol measures by HPLC (R(2) = 0.82). CONCLUSIONS: The RBP EIA is as reliable in estimating VAD as is HPLC retinol. After successful validations, the test should enable public health authorities to rapidly monitor VAD and track vitamin A status in populations. |
| | |
Authors:
|
John Hix; Carolina Martinez; Ian Buchanan; Jeff Morgan; Milton Tam; Anuraj Shankar |
Related Documents
:
|
3509183 - Analysis of the interacting components between larval schistosoma mansoni and schistoso... 1801313 - Natural anti-snake venom proteins. 18989393 - Transmembrane topology and signal peptide prediction using dynamic bayesian networks. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: The American journal of clinical nutrition Volume: 79 ISSN: 0002-9165 ISO Abbreviation: Am. J. Clin. Nutr. Publication Date: 2004 Jan |
Date Detail:
|
Created Date: 2003-12-19 Completed Date: 2004-01-20 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0376027 Medline TA: Am J Clin Nutr Country: United States |
Other Details:
|
Languages: eng Pagination: 93-8 Citation Subset: AIM; IM |
Affiliation:
|
Program for Appropriate Technology in Health, Seattle, WA 98107, USA. jhix@path.org |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Child Chromatography, High Pressure Liquid Female Humans Immunoenzyme Techniques / methods* Reagent Strips Reproducibility of Results Retinol-Binding Proteins / metabolism* Vitamin A / blood* |
| Chemical | |
Reg. No./Substance:
|
0/Reagent Strips; 0/Retinol-Binding Proteins; 11103-57-4/Vitamin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Vitamin E bioavailability from fortified breakfast cereal is greater than that from encapsulated sup...
Next Document: Erythorbic acid is a potent enhancer of nonheme-iron absorption.