Document Detail


Development of a radioimmunoassay for theophylline. Application to studies in premature infants.
MedLine Citation:
PMID:  558079     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of theophylline in premature infants has necessitated the development of an assay applicable to samples under 0.1 ml. To accomplish this we have developed a radioimmunoassay for theophylline. The antiserum to theophylline has been characterized and found to be highly specific for theophylline and sensitive to 2 ng. The affinity of our antibody and the titer developed in the goat allowed the assay to be performed routinely with the use of a plasma sample size of only 2 to 5 micronl. Theophylline levels of 6 to 20 microng/ml were found 2 hr after maintenance doses of aminophylline, 4 mg/kg per rectum in 12 premature infants. Terminal serum half-lives ranged from 12 to 54 hr.
Authors:
A L Neese; L F Soyka
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical pharmacology and therapeutics     Volume:  21     ISSN:  0009-9236     ISO Abbreviation:  Clin. Pharmacol. Ther.     Publication Date:  1977 May 
Date Detail:
Created Date:  1977-06-30     Completed Date:  1977-06-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372741     Medline TA:  Clin Pharmacol Ther     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  633-41     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Aminophylline / metabolism,  therapeutic use
Animals
Antibody Specificity
Apnea / blood,  drug therapy
Binding, Competitive
Cross Reactions
Goats / immunology
Humans
Infant, Newborn
Infant, Premature, Diseases / blood*,  drug therapy
Methods
Radioimmunoassay
Theophylline / blood*
Time Factors
Chemical
Reg. No./Substance:
317-34-0/Aminophylline; 58-55-9/Theophylline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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