Document Detail

Development of a radioimmunoassay for the measurement of Bisphenol A in biological samples.
MedLine Citation:
PMID:  19481623     Owner:  NLM     Status:  MEDLINE    
Bisphenol A (BPA) is widely used in the manufacturing of polycarbonate plastic food and drink packaging. Possessing a weak estrogenic activity, BPA is listed among a growing list of endocrine disrupting compounds. In this study, a polyclonal anti-BPA antibody was obtained by immunization with BPA-monocarboxymethylether covalently linked to BSA. The antibody demonstrates negligible cross-reactivity with most analogous BPA phenolic structures, and no cross-reactivity with endogenous steroids. An extraction step with ethyl acetate minimized matrix effects and allowed the BPA measurement in plasma and other biological samples. Recovery after loading test was 96 +/- 4% and dilution tests had a linear profile (r2 > 0.93). The limit of detection of the BPA RIA was 0.08 microg L(-1) with an IC50 of 1.25 microg L(-1). The intra- and inter-assay coefficients of variation were 5.6 and 8.6%, respectively at a BPA concentration of 0.7 microg L(-1) and 6.9 and 5.7% at a BPA concentration of 1.3 microg L(-1). A significant correlation was found between the values obtained by the RIA and HPLC-MS (r2 = 0.92) or HPLC coupled to a fluorescence detector (r2 = 0.80). In conclusion, we described a BPA-RIA that is a suitable tool for evaluating human exposure to BPA.
Nisrin Kaddar; Nadia Bendridi; Catherine Harthé; Marc Rolland de Ravel; Anne-Lise Bienvenu; Claude-Yves Cuilleron; Elisabeth Mappus; Michel Pugeat; Henri Déchaud
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2009-05-03
Journal Detail:
Title:  Analytica chimica acta     Volume:  645     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-01     Completed Date:  2009-08-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-4     Citation Subset:  IM    
Université de Lyon 1 ISBP, France.
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MeSH Terms
Antibodies / immunology
Cross Reactions
Follicular Fluid / chemistry
Phenols / analysis*,  blood,  immunology*
Radioimmunoassay / economics,  methods*
Sample Size
Semen / chemistry
Sensitivity and Specificity
Reg. No./Substance:
0/Antibodies; 0/Phenols; 80-05-7/bisphenol A

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