| Development of a radioimmunoassay for the measurement of Bisphenol A in biological samples. | |
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MedLine Citation:
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PMID: 19481623 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bisphenol A (BPA) is widely used in the manufacturing of polycarbonate plastic food and drink packaging. Possessing a weak estrogenic activity, BPA is listed among a growing list of endocrine disrupting compounds. In this study, a polyclonal anti-BPA antibody was obtained by immunization with BPA-monocarboxymethylether covalently linked to BSA. The antibody demonstrates negligible cross-reactivity with most analogous BPA phenolic structures, and no cross-reactivity with endogenous steroids. An extraction step with ethyl acetate minimized matrix effects and allowed the BPA measurement in plasma and other biological samples. Recovery after loading test was 96 +/- 4% and dilution tests had a linear profile (r2 > 0.93). The limit of detection of the BPA RIA was 0.08 microg L(-1) with an IC50 of 1.25 microg L(-1). The intra- and inter-assay coefficients of variation were 5.6 and 8.6%, respectively at a BPA concentration of 0.7 microg L(-1) and 6.9 and 5.7% at a BPA concentration of 1.3 microg L(-1). A significant correlation was found between the values obtained by the RIA and HPLC-MS (r2 = 0.92) or HPLC coupled to a fluorescence detector (r2 = 0.80). In conclusion, we described a BPA-RIA that is a suitable tool for evaluating human exposure to BPA. |
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Authors:
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Nisrin Kaddar; Nadia Bendridi; Catherine Harthé; Marc Rolland de Ravel; Anne-Lise Bienvenu; Claude-Yves Cuilleron; Elisabeth Mappus; Michel Pugeat; Henri Déchaud |
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Publication Detail:
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Type: Evaluation Studies; Journal Article Date: 2009-05-03 |
Journal Detail:
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Title: Analytica chimica acta Volume: 645 ISSN: 1873-4324 ISO Abbreviation: Anal. Chim. Acta Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-06-01 Completed Date: 2009-08-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370534 Medline TA: Anal Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-4 Citation Subset: IM |
Affiliation:
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Université de Lyon 1 ISBP, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / immunology Cattle Cross Reactions Female Follicular Fluid / chemistry Humans Male Phenols / analysis*, blood, immunology* Radioimmunoassay / economics, methods* Sample Size Semen / chemistry Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Phenols; 80-05-7/bisphenol A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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