Document Detail


Development and polarization of cationic amino acid transporters and regulators in the human placenta.
MedLine Citation:
PMID:  10837344     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have investigated L-arginine transport systems in the human placental syncytiotrophoblast across gestation using purified microvillous (MVM) and basal (BM) plasma membrane vesicles. In MVM from first-trimester and term placentas, L-arginine transport was by systems y(+) and y(+)L. In BM (term placentas), however, there was evidence for system y(+)L only. The Michaelis constant of system y(+)L was significantly lower (P < 0.05) in first-trimester compared with term MVM and lower in term MVM compared with BM (P < 0.05). There was no functional evidence for system b(0+) in term MVM or BM. Cationic amino acid transporter (CAT) 1, CAT 4, and 4F2hc were detected using RT-PCR in placentas throughout gestation. rBAT was not detected in term placentas. An approximately 85-kDa and an approximately 135-kDa protein was detected by Western blotting in MVM under reducing and nonreducing conditions, respectively, consistent with the 4F2hc monomer and the 4F2hc-light chain dimer, and their expression was significantly higher (P < 0.05) in term compared with first-trimester MVM. These proteins were not detected in BM despite functional evidence for system y(+)L. These data suggest different roles for 4F2hc in the development and polarization of cationic amino acid transporters in the syncytiotrophoblast.
Authors:
P T Ayuk; C P Sibley; P Donnai; S D'Souza; J D Glazier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  278     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-07-18     Completed Date:  2000-07-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C1162-71     Citation Subset:  IM    
Affiliation:
Academic Unit of Child Health, University of Manchester and Department of Obstetrics and Gynecology, St. Mary's Hospital, Manchester M13 0JH, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Transport Systems, Basic
Amino Acids / metabolism,  pharmacology
Antigens, CD / genetics*,  metabolism
Antigens, CD98
Biological Transport / drug effects
Carrier Proteins / genetics*,  metabolism
Chorionic Villi / physiology*
Delivery, Obstetric
Female
Gene Expression Regulation*
Humans
Kinetics
Membrane Glycoproteins / genetics*,  metabolism
Membrane Proteins / genetics*,  metabolism
Placenta / physiology*
Pregnancy
Pregnancy Trimester, First
Reverse Transcriptase Polymerase Chain Reaction
Trophoblasts / physiology*
Chemical
Reg. No./Substance:
0/Amino Acid Transport Systems, Basic; 0/Amino Acids; 0/Antigens, CD; 0/Antigens, CD98; 0/Carrier Proteins; 0/Membrane Glycoproteins; 0/Membrane Proteins; 0/SLC7A9 protein, human

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