| Development of a piezoelectric immunosensor for the detection of Salmonella typhimurium. | |
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MedLine Citation:
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PMID: 1366514 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A piezoelectric biosensor has been developed for the detection of Salmonella typhimurium. The antibody to Salmonella was immobilized on the crystal by various immobilization procedures. The best result was obtained when antibody was immobilized on the crystal precoated with a thin layer of polyethyleneimine. The response of the coated crystal for S. typhimurium in a microbial suspension was in the range of 10(5) to 10(9) cells ml-1. The time required for a complete interaction between the crystal and the cells appeared to depend upon the cell concentration of the analyzed sample. The antibody-bound crystal lost no activity over 4 days at 4 degrees C and it could be reused for 6-8 consecutive assays. |
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Authors:
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E Prusak-Sochaczewski; J H Luong; G G Guilbault |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Enzyme and microbial technology Volume: 12 ISSN: 0141-0229 ISO Abbreviation: Enzyme Microb. Technol. Publication Date: 1990 Mar |
Date Detail:
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Created Date: 1990-09-04 Completed Date: 1990-09-04 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 8003761 Medline TA: Enzyme Microb Technol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 173-7 Citation Subset: B |
Affiliation:
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National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Bacterial Biosensing Techniques* Electrodes Food Microbiology* Indicators and Reagents Methods Salmonella typhimurium / isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Indicators and Reagents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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