Document Detail

Development of a photodiode array biochip using a bipolar semiconductor and its application to detection of human papilloma virus.
MedLine Citation:
PMID:  18193408     Owner:  NLM     Status:  MEDLINE    
We describe a DNA microarray system using a bipolar integrated circuit photodiode array (PDA) chip as a new platform for DNA analysis. The PDA chip comprises an 8 x 6 array of photodiodes each with a diameter of 600 microm. Each photodiode element acts both as a support for an immobilizing probe DNA and as a two-dimensional photodetector. The usefulness of the PDA microarray platform is demonstrated by the detection of high-risk subtypes of human papilloma virus (HPV). The polymerase chain reaction (PCR)-amplified biotinylated HPV target DNA was hybridized with the immobilized probe DNA on the photodiode surface, and the chip was incubated in an anti-biotin antibody-conjugated gold nanoparticle solution. The silver enhancement by the gold nanoparticles bound to the biotin of the HPV target DNA precipitates silver metal particles at the chip surfaces, which block light irradiated from above. The resulting drop in output voltage depends on the amount of target DNA present in the sample solution, which allows the specific detection and the quantitative analysis of the complementary target DNA. The PDA chip showed high relative signal ratios of HPV probe DNA hybridized with complementary target DNA, indicating an excellent capability in discriminating HPV subtypes. The detection limit for the HPV target DNA analysis improved from 1.2 nM to 30 pM by changing the silver development time from 5 to 10 min. Moreover, the enhanced silver development promoted by the gold nanoparticles could be applied to a broader range of target DNA concentration by controlling the silver development time.
Taek Jin Baek; Pan Yun Park; Kwi Nam Han; Ho Taik Kwon; Gi Hun Seong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-13
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  390     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-26     Completed Date:  2008-04-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1373-8     Citation Subset:  IM    
Department of Applied Chemistry, Hanyang University, Sa-1 dong 1271, Ansan, 425-791, South Korea.
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MeSH Terms
DNA, Viral / genetics
Microscopy, Electron, Scanning
Oligonucleotide Array Sequence Analysis / methods*
Papillomaviridae / genetics*,  ultrastructure
Reg. No./Substance:
0/DNA, Viral

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