| Development and performance evaluation of calf diarrhea pathogen nucleic acid purification and detection workflow. | |
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MedLine Citation:
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PMID: 22914823 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Calf diarrhea (scours) is a primary cause of illness and death in young calves. Significant economic losses associated with this disease include morbidity, mortality, and direct cost of treatment. Multiple pathogens are responsible for infectious diarrhea, including, but not limited to, Bovine coronavirus (BCV), bovine Rotavirus A (BRV), and Cryptosporidium spp. Identification and isolation of carrier calves are essential for disease management. Texas Veterinary Medical Diagnostic Laboratory current methods for calf diarrhea pathogen identification include electron microscopy (EM) for BCV and BRV and a direct fluorescent antibody test (DFAT) for organism detection of Cryptosporidium spp. A workflow was developed consisting of an optimized fecal nucleic acid purification and multiplex reverse transcription quantitative polymerase chain reaction (RT-qPCR) for single tube concurrent detection of BCV, BRV, and Cryptosporidium spp., and an internal control to monitor nucleic acid purification efficacy and PCR reagent functionality. In "spike-in" experiments using serial dilutions of each pathogen, the analytical sensitivity was determined to be <10 TCID(50)/ml for BCV and BRV, and <20 oocysts for Cryptosporidium spp. Analytical specificity was confirmed using Canine and Feline coronavirus, Giardia spp., and noninfected bovine purified nucleic acid. Diagnostic sensitivity was ≥98% for all pathogens when compared with respective traditional methods. The results demonstrate that the newly developed assay can purify and subsequently detect BCV, BRV, and Cryptosporidium spp. concurrently in a single PCR, enabling simplified and streamlined calf diarrhea pathogen identification. |
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Authors:
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Megan E Schroeder; Mangkey A Bounpheng; Sandy Rodgers; Rocky J Baker; Wendy Black; Hemant Naikare; Binu Velayudhan; Loyd Sneed; Barbara Szonyi; Alfonso Clavijo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc Volume: 24 ISSN: 1943-4936 ISO Abbreviation: J. Vet. Diagn. Invest. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-08-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9011490 Medline TA: J Vet Diagn Invest Country: United States |
Other Details:
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Languages: eng Pagination: 945-53 Citation Subset: IM |
Affiliation:
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1Mangkey A. Bounpheng, Texas Veterinary Medical Diagnostic Laboratory, 1 Sippel Road, College Station, TX 77843. mbounpheng@tvmdl.tamu.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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