| Development of an oligonucleotide-specific capture plate hybridization assay for detection of Haemophilus parasuis. | |
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MedLine Citation:
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PMID: 10098685 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An oligonucleotide-specific capture plate hybridization assay has been developed to rapidly, specifically, and sensitively detect Haemophilus parasuis from nasal swabs. Several in vitro studies have been performed to determine the sensitivity and specificity of the test, and in vivo studies have validated this technique in pigs. Results suggest that the assay detects <100 colony-forming units/ml in a pure culture and gives a positive result when H. parasuis is present in a ratio of 1:10(3)-10(4) in a mixed culture, and the probe does not hybridize with other related species found in the upper respiratory tract. This assay is more sensitive than culture for detection of the microorganism from nasal swabs and lesions. |
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Authors:
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M Calsamiglia; C Pijoan; G Solano; V Rapp-Gabrielson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc Volume: 11 ISSN: 1040-6387 ISO Abbreviation: J. Vet. Diagn. Invest. Publication Date: 1999 Mar |
Date Detail:
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Created Date: 1999-05-17 Completed Date: 1999-05-17 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9011490 Medline TA: J Vet Diagn Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 140-5 Citation Subset: IM |
Affiliation:
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Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals DNA, Bacterial / analysis* Haemophilus Infections / diagnosis* Molecular Sequence Data Nasal Cavity / microbiology Oligonucleotide Array Sequence Analysis / methods, veterinary* Sensitivity and Specificity Swine Swine Diseases / diagnosis, microbiology* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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