| Development of a novel pretargeting system with bifunctional nucleic acid molecules. | |
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MedLine Citation:
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PMID: 19545539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study was aimed at exploring a novel pretargeting system based upon bifunctional nucleic acid molecules that are comprised of a nucleic acid aptamer and a nucleic acid tail. The properties of bifunctional molecules were investigated by both theoretical prediction and experimental determination. Different from the algorithm-based structure prediction, the experimental data showed that some nucleic acid tails could significantly decrease the binding capability of the aptamer. It was also found that the effectiveness of bifunctional molecules in labeling cells was dependent on the hybridization length. Based on these understandings, one bifunctional molecule was selected to study pretargeting. The results demonstrated that the bifunctional molecule could not only bind to target cells, but also hybridize with its complementary oligonucleotide on the cell surface. Thus, bifunctional nucleic acid molecules hold great potential for pretargeting applications. |
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Authors:
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Jing Zhou; Boonchoy Soontornworajit; Matthew P Snipes; Yong Wang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-06-21 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 386 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-17 Completed Date: 2009-08-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 521-5 Citation Subset: IM |
Affiliation:
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Department of Chemical, Materials & Biomolecular Engineering, University of Connecticut, Storrs, CT 06269, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aptamers, Nucleotide
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chemistry* Cell Line, Tumor DNA / chemistry* Drug Delivery Systems* Humans |
| Chemical | |
Reg. No./Substance:
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0/Aptamers, Nucleotide; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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