Document Detail

Development of a novel noninvasive adhesive patch test for the evaluation of pigmented lesions of the skin.
MedLine Citation:
PMID:  24906614     Owner:  NLM     Status:  Publisher    
BACKGROUND: The accurate clinical assessment of melanocytic neoplasms is a challenge for clinicians. Currently, obtaining a biopsy specimen and conducting a histologic examination is the standard of care. The incidence of melanoma in white populations is high, resulting in a large number of biopsy specimens.
OBJECTIVE: The objective of this study is to develop a noninvasive genomic method using mRNA to classify pigmented skin lesions as either benign or malignant.
METHODS: An adhesive patch method was used to obtain cells from the surface of melanocytic lesions. mRNA was extracted and a genomic signature was formulated in a training set of benign and malignant melanocytic neoplasms and subsequently tested in a validation set.
RESULTS: A 2-gene signature assessing the expression levels of CMIP and LINC00518 was able to differentiate melanomas from nevi in an independent validation set of 42 melanomas and 22 nevi with a sensitivity of 97.6% and specificity of 72.7%.
LIMITATIONS: Larger and more diverse sets of melanomas and nevi are needed for additional validation of the molecular expression profiling in various subsets of melanocytic neoplasms.
CONCLUSION: Our data suggest that mRNA molecular signatures can serve as a highly useful noninvasive method of differentiating melanoma from nevi and decrease the number of unnecessary biopsies.
Pedram Gerami; John P Alsobrook; Tara J Palmer; Howard S Robin
Related Documents :
9658254 - Skin problems in the traveler.
23698754 - An exploratory research of the endobronchial ultrasonography model to estimate the prob...
24726734 - The components of progression as explanatory variables for overall survival in the resp...
25073734 - Relationship between melanoma detection pattern and tumor thickness.
21508404 - Concomitant diagnosis of myeloproliferative neoplasm and non-hodgkin's lymphoma in a pa...
19357914 - Laparoscopic ultrasound-guided biopsy in upper gastrointestinal tract cancer patients.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-6-3
Journal Detail:
Title:  Journal of the American Academy of Dermatology     Volume:  -     ISSN:  1097-6787     ISO Abbreviation:  J. Am. Acad. Dermatol.     Publication Date:  2014 Jun 
Date Detail:
Created Date:  2014-6-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7907132     Medline TA:  J Am Acad Dermatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014 American Academy of Dermatology, Inc. Published by Mosby, Inc. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Probiotics and prebiotics in dermatology.
Next Document:  Efficacy and safety of felbamate in children with refractory epilepsy.