| Development of a novel method for operating magnetic particles, Magtration Technology, and its use for automating nucleic acid purification. | |
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MedLine Citation:
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PMID: 16233029 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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Magnetic particles are useful for simple and efficient nucleic acid extraction. To achieve fully automated nucleic acid extraction and purification using magnetic particles, a new method for operating magnetic particles, Magtration Technology, was developed. In this method, magnetic separation is performed in a specially designed disposable tip. This enables high recovery of magnetic particles with high reproducibility. The features of this technology are (i) a simple mechanism for process control and (ii) flexible software to enable adaptation to commercially available reagents. Automated instruments based on Magtration Technology were developed and used for nucleic acid extraction. Total DNA, total RNA and plasmids were purified by Magtration Technology at an efficiency comparable to that of manual methods. |
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Authors:
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K Obata; O Segawa; M Yakabe; Y Ishida; T Kuroita; K Ikeda; B Kawakami; Y Kawamura; M Yohda; T Matsunaga; H Tajima |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bioscience and bioengineering Volume: 91 ISSN: 1389-1723 ISO Abbreviation: J. Biosci. Bioeng. Publication Date: 2001 |
Date Detail:
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Created Date: 2005-10-19 Completed Date: 2005-11-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888800 Medline TA: J Biosci Bioeng Country: Japan |
Other Details:
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Languages: eng Pagination: 500-3 Citation Subset: - |
Affiliation:
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Precision System Science Co. Ltd., 88 Kamihongo, Matsudo, Chiba 271-0064, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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