Document Detail


Development of a novel method for operating magnetic particles, Magtration Technology, and its use for automating nucleic acid purification.
MedLine Citation:
PMID:  16233029     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Magnetic particles are useful for simple and efficient nucleic acid extraction. To achieve fully automated nucleic acid extraction and purification using magnetic particles, a new method for operating magnetic particles, Magtration Technology, was developed. In this method, magnetic separation is performed in a specially designed disposable tip. This enables high recovery of magnetic particles with high reproducibility. The features of this technology are (i) a simple mechanism for process control and (ii) flexible software to enable adaptation to commercially available reagents. Automated instruments based on Magtration Technology were developed and used for nucleic acid extraction. Total DNA, total RNA and plasmids were purified by Magtration Technology at an efficiency comparable to that of manual methods.
Authors:
K Obata; O Segawa; M Yakabe; Y Ishida; T Kuroita; K Ikeda; B Kawakami; Y Kawamura; M Yohda; T Matsunaga; H Tajima
Related Documents :
15649809 - Determination of nucleic acids based on shifting the association equilibrium between te...
16386949 - The sensitive determination of nucleic acids using resonance light scattering quenching...
6541899 - Studies on simian hemorrhagic fever virus nucleocapsids.
2729579 - Synthesis of 5'-oligonucleotide hydrazide derivatives and their use in preparation of e...
15347259 - In situ hybridization: detecting viral nucleic acid in formalin-fixed, paraffin-embedde...
1416029 - Immunochemical detection of proteins and nucleic acids on filters using small luminesce...
16840019 - Nutrient utilisation in muscle and in the whole body of patients receiving total parent...
8446599 - Roles of ser101, asp236, and his237 in catalysis of thioesterase ii and of the c-termin...
806609 - The development of essential fatty acid deficiency in healthy men fed fat-free diets in...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  91     ISSN:  1389-1723     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2001  
Date Detail:
Created Date:  2005-10-19     Completed Date:  2005-11-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  500-3     Citation Subset:  -    
Affiliation:
Precision System Science Co. Ltd., 88 Kamihongo, Matsudo, Chiba 271-0064, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Efficient removal of sulfide following integration of multiple copies of the sulfide-quinone oxidore...
Next Document:  Optimization of tylosin feeding rate profile in production of acetyl-isovaleryl tylosin (AIV) from t...