| Development of a new cytomegalovirus (CMV) immunoglobulin M (IgM) immunoblot for detection of CMV-specific IgM. | |
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MedLine Citation:
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PMID: 9774589 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We developed a new cytomegalovirus (CMV) immunoglobulin M (IgM) immunoblot to detect CMV-specific IgM in human sera. The new test contains four viral proteins (vp150, vp82, vp65, and vp28) purified from viral particles and four recombinant proteins (rp150, rp130, rp52, and rp38) purified from Escherichia coli. These antigens were individually loaded onto nitrocellulose strips, and the strips were then used to detect CMV-specific IgM by using a mu-specific conjugate. The new assay was evaluated in parallel with one or two IgM enzyme-linked immunosorbent assays (ELISAs) to test 592 serum samples from different groups of latently or acutely infected individuals. The sensitivity of the new assay with respect to the consensus of two ELISAs was 100%, the specificity was 98.6%, the positive predictive value was 96.9%, and the negative predictive value was 100%. We also evaluated the new test by testing sera from pregnant women and transplant recipients with a known clinical history. Our results suggest that the new test combines high sensitivity with high specificity, characteristics that are mutually exclusive with the other commercially available tests. Furthermore, a statistically significant correlation was observed between the number of IgM-reactive bands and the elevated risk of transmission from CMV-infected pregnant women to their offspring. |
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Authors:
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T Lazzarotto; A Ripalti; G Bergamini; M C Battista; P Spezzacatena; F Campanini; P Pradelli; S Varani; L Gabrielli; G T Maine; M P Landini |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 36 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 1998 Nov |
Date Detail:
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Created Date: 1998-12-11 Completed Date: 1998-12-11 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3337-41 Citation Subset: IM |
Affiliation:
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Department of Clinical and Experimental Medicine, Section of Microbiology, University of Bologna, Bologna, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Algorithms Antibodies, Viral / blood* Antibody Specificity Antigens, Viral Blotting, Western / methods* Cytomegalovirus / immunology* Cytomegalovirus Infections / complications, diagnosis*, immunology* Evaluation Studies as Topic Female Humans Immunoglobulin M / blood* Maternal-Fetal Exchange Organ Transplantation Pregnancy Pregnancy Complications, Infectious / diagnosis, immunology Recombinant Proteins / immunology Viral Proteins / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Viral; 0/Antigens, Viral; 0/Immunoglobulin M; 0/Recombinant Proteins; 0/Viral Proteins |
| Comments/Corrections | |
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