Document Detail


Development of a new cytomegalovirus (CMV) immunoglobulin M (IgM) immunoblot for detection of CMV-specific IgM.
MedLine Citation:
PMID:  9774589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We developed a new cytomegalovirus (CMV) immunoglobulin M (IgM) immunoblot to detect CMV-specific IgM in human sera. The new test contains four viral proteins (vp150, vp82, vp65, and vp28) purified from viral particles and four recombinant proteins (rp150, rp130, rp52, and rp38) purified from Escherichia coli. These antigens were individually loaded onto nitrocellulose strips, and the strips were then used to detect CMV-specific IgM by using a mu-specific conjugate. The new assay was evaluated in parallel with one or two IgM enzyme-linked immunosorbent assays (ELISAs) to test 592 serum samples from different groups of latently or acutely infected individuals. The sensitivity of the new assay with respect to the consensus of two ELISAs was 100%, the specificity was 98.6%, the positive predictive value was 96.9%, and the negative predictive value was 100%. We also evaluated the new test by testing sera from pregnant women and transplant recipients with a known clinical history. Our results suggest that the new test combines high sensitivity with high specificity, characteristics that are mutually exclusive with the other commercially available tests. Furthermore, a statistically significant correlation was observed between the number of IgM-reactive bands and the elevated risk of transmission from CMV-infected pregnant women to their offspring.
Authors:
T Lazzarotto; A Ripalti; G Bergamini; M C Battista; P Spezzacatena; F Campanini; P Pradelli; S Varani; L Gabrielli; G T Maine; M P Landini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  36     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1998-12-11     Completed Date:  1998-12-11     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3337-41     Citation Subset:  IM    
Affiliation:
Department of Clinical and Experimental Medicine, Section of Microbiology, University of Bologna, Bologna, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adult
Algorithms
Antibodies, Viral / blood*
Antibody Specificity
Antigens, Viral
Blotting, Western / methods*
Cytomegalovirus / immunology*
Cytomegalovirus Infections / complications,  diagnosis*,  immunology*
Evaluation Studies as Topic
Female
Humans
Immunoglobulin M / blood*
Maternal-Fetal Exchange
Organ Transplantation
Pregnancy
Pregnancy Complications, Infectious / diagnosis,  immunology
Recombinant Proteins / immunology
Viral Proteins / immunology
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Antigens, Viral; 0/Immunoglobulin M; 0/Recombinant Proteins; 0/Viral Proteins
Comments/Corrections

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