Document Detail

Development of the myoglobin sensor based on the surface plasmon resonance.
MedLine Citation:
PMID:  10609299     Owner:  NLM     Status:  MEDLINE    
Bioaffinic sensor based on the surface plasmon resonance (SPR) with golden layer as a transducer was developed. This sensor was applied for control of antigen-antibodies (Ag-Ab) interactions. For modification of transducer surface the spontaneously organized molecular assemblies of two types of mercaptan were formed. It was shown that the created bioaffinic sensor allowed to detect minimal concentration of antibodies, about 1 ng/ml, to the human heart myoglobin, which preliminary was immobilized on the sensitive surface. The particularities of the dynamics of protein interaction were observed. Duration of incubation with the biospecific component was no more than 5 minutes. After rupturing of specific binding it was possible to obtain initial signal again during 7-9 cycles.
N F Starodub; T L Dibrova; Shirshov YuM; K V Kostyukevych
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Ukrainskiĭ biokhimicheskiĭ zhurnal     Volume:  71     ISSN:  0201-8470     ISO Abbreviation:  Ukr. Biokhim. Zh.     Publication Date:    1999 Mar-Apr
Date Detail:
Created Date:  2000-02-01     Completed Date:  2000-02-01     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7804246     Medline TA:  Ukr Biokhim Zh     Country:  UKRAINE    
Other Details:
Languages:  eng     Pagination:  33-7     Citation Subset:  IM    
A.V. Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv.
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MeSH Terms
Biosensing Techniques*
Myocardium / chemistry
Myoglobin / analysis*
Surface Plasmon Resonance
Reg. No./Substance:

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