Document Detail


Development of a multiplex quantitative fluorescent PCR assay for identification of rearrangements in the AZFb and AZFc regions.
MedLine Citation:
PMID:  18445645     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The azoospermia factor b (AZFb) and azoospermia factor c (AZFc) regions in the human Y chromosome consist of five palindromes constructed from six distinct families of amplicons and are prone to rearrangement. Partial deletion and duplication in the region can cause azoospermia or oligozoospermia and male infertility. The aim of the study was to establish a quantitative fluorescent PCR (QF-PCR) assay to classify AZFb and AZFc rearrangements. A single pair of fluorescent primers was designed to amplify simultaneously the amplicon in AZFc and the length-variant homologous sequences outside of the region as control. Since the copy number of the control sequences is fixed in the human genome, dosage of the target could be easily obtained through comparing the height of the fluorescent peaks between the target and the control after amplification with limited PCR cycles. Most types of rearrangements in AZFb and AZFc regions could be classified with QF-PCR containing four such primer pairs. Eleven types of rearrangement in AZFb and AZFc regions were well discriminated with QF-PCR. In conclusion, QF-PCR is a simple and reliable method to detect rearrangements in AZFb and AZFc.
Authors:
Jun Zhang; Pei-qiong Li; Qi-hong Yu; Hua-yun Chen; Juan Li; Yun-shao He
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2008-04-29
Journal Detail:
Title:  Molecular human reproduction     Volume:  14     ISSN:  1460-2407     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-23     Completed Date:  2008-08-19     Revised Date:  2011-12-14    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  371-6     Citation Subset:  IM    
Affiliation:
Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Chromosomes, Human, Y*
Female
Fluorescent Dyes* / pharmacology
Gene Deletion
Gene Dosage
Genetic Loci
Humans
Infertility / genetics
Male
Models, Biological
Polymerase Chain Reaction / methods*
Seminal Plasma Proteins / genetics*
Sex Chromosome Aberrations*
XYY Karyotype
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Seminal Plasma Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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