Document Detail


Development of a multiple-run high-resolution melting assay for Salmonella spp. genotyping HRM application for Salmonella spp. subtyping.
MedLine Citation:
PMID:  21996359     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A quick and robust Salmonella spp. differentiation method based on high-resolution DNA melting (HRM) was developed. DNA samples from 134 Salmonella spp. strains and 20 serotypes were tested. Each serotype was represented by at least 2 strains. All raw data were derived on the Rotor-Gene 65H0-100 system using the designed 8 primer pairs. The reference samples for HRM error evaluation between runs were applied. Raw data error minimization and fluorescence normalization between runs were carried out by application of the proposed calculations. The data analysis showed that repetitive sequence targets are much more informative than the nonrepetitive ones. The method possesses a high potential and can be adopted for further subtyping analyses.
Authors:
Maksim Bratchikov; Mykolas Mauricas
Related Documents :
22262099 - Detoxification of toxins by bacillithiol in staphylococcus aureus.
22092439 - Listeria monocytogenes tyrosine phosphatases affect wall teichoic acid composition and ...
21899579 - Significance of the rdar and bdar morphotypes in the hydrophobicity and attachment to a...
22975569 - Necrotizing pneumonia in the community.
23300299 - Occurrence of vancomycin-resistant enterococci in turkey flocks.
21262989 - Wood decomposing abilities of diverse lignicolous fungi on nondecayed and decayed beech...
9828439 - Bacillus weihenstephanensis sp. nov. is a new psychrotolerant species of the bacillus c...
19648339 - Polynucleobacter cosmopolitanus sp. nov., free-living planktonic bacteria inhabiting fr...
15066799 - Characterization of ferroplasma isolates and ferroplasma acidarmanus sp. nov., extreme ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diagnostic microbiology and infectious disease     Volume:  71     ISSN:  1879-0070     ISO Abbreviation:  Diagn. Microbiol. Infect. Dis.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-10-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8305899     Medline TA:  Diagn Microbiol Infect Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  192-200     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Stabilizing moments of force on a prosthetic knee during stance in the first steps after gait initia...
Next Document:  Comparison of interferon gamma-inducible protein-10 and interferon gamma-based QuantiFERON TB Gold a...