| Development of a minimal defined medium for recombinant human interleukin-3 production by Streptomyces lividans 66. | |
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MedLine Citation:
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PMID: 17615562 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A systematic approach was developed to identify and optimize the essential amino acids in defined minimal medium for the production of recombinant human interleukin 3 (rHuIL-3) by Streptomyces lividans. Starvation trials were carried out initially to narrow down the number of probable essential amino acids from an initial number of 20 to 8. Then a screening mixture experiment was designed and performed with the eight identified amino acids and distance-based multivariate analysis was employed to rank the probable essential amino acids regarding both growth and product formation. Following this procedure, the search was narrowed to four amino acids (Asp, Leu, Met, and Phe). Finally, a mixture design experiment known as the simplex lattice design was carried out and the composition of the optimum minimal medium was found (Asp 53%, Met 5%, and Phe 42%). |
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Authors:
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Keyvan Nowruzi; Ali Elkamel; Jeno M Scharer; Doug Cossar; Murray Moo-Young |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 99 ISSN: 1097-0290 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2007-12-06 Completed Date: 2008-01-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 214-22 Citation Subset: IM |
Copyright Information:
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(c) 2007 Wiley Periodicals, Inc. |
Affiliation:
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Department of Chemical Engineering, University of Waterloo, Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Culture Techniques
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methods* Cell Proliferation Cell Survival Culture Media / metabolism* Humans Interleukin-3 / genetics, metabolism* Protein Engineering / methods* Recombinant Proteins / metabolism Streptomyces lividans / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Interleukin-3; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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