Document Detail

Development of a minimal defined medium for recombinant human interleukin-3 production by Streptomyces lividans 66.
MedLine Citation:
PMID:  17615562     Owner:  NLM     Status:  MEDLINE    
A systematic approach was developed to identify and optimize the essential amino acids in defined minimal medium for the production of recombinant human interleukin 3 (rHuIL-3) by Streptomyces lividans. Starvation trials were carried out initially to narrow down the number of probable essential amino acids from an initial number of 20 to 8. Then a screening mixture experiment was designed and performed with the eight identified amino acids and distance-based multivariate analysis was employed to rank the probable essential amino acids regarding both growth and product formation. Following this procedure, the search was narrowed to four amino acids (Asp, Leu, Met, and Phe). Finally, a mixture design experiment known as the simplex lattice design was carried out and the composition of the optimum minimal medium was found (Asp 53%, Met 5%, and Phe 42%).
Keyvan Nowruzi; Ali Elkamel; Jeno M Scharer; Doug Cossar; Murray Moo-Young
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  99     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-06     Completed Date:  2008-01-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  214-22     Citation Subset:  IM    
Copyright Information:
(c) 2007 Wiley Periodicals, Inc.
Department of Chemical Engineering, University of Waterloo, Waterloo, 200 University Avenue West, Waterloo, Ontario, Canada.
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MeSH Terms
Cell Culture Techniques / methods*
Cell Proliferation
Cell Survival
Culture Media / metabolism*
Interleukin-3 / genetics,  metabolism*
Protein Engineering / methods*
Recombinant Proteins / metabolism
Streptomyces lividans / genetics,  metabolism*
Reg. No./Substance:
0/Culture Media; 0/Interleukin-3; 0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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