| Development of a microplate fluorescence assay for kynurenine aminotransferase. | |
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MedLine Citation:
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PMID: 21059337 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Inhibition of kynurenine aminotransferases (KATs) is a strategy to therapeutically reduce levels of kynurenic acid (KYNA), an endogenous antagonist of glutamatergic N-methyl-D-aspartate (NMDA) and cholinergic α₇ nicotinic receptors. Several methods of measuring KAT activity in vitro have been developed, but none is well-suited to high throughput and automation. In this article, we describe a modification of existing high-performance liquid chromatography (HPLC)-based methods that enables the development of a 96-well microplate assay in both enzyme- and cell-based formats using human KAT I as an example. KYNA enzymatically produced from L-kynurenine is measured directly in a reaction mixture fluorimetrically. |
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Authors:
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Jacky Wong; William J Ray; Anna Y Kornilova |
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Publication Detail:
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Type: Journal Article Date: 2010-11-06 |
Journal Detail:
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Title: Analytical biochemistry Volume: 409 ISSN: 1096-0309 ISO Abbreviation: Anal. Biochem. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2010-12-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 183-8 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Neurology Department, Merck, West Point, PA 19486, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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