Document Detail


Development of a micro cell compression stimulator for evaluating real-time cellular responses.
MedLine Citation:
PMID:  22667645     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
This paper presents a micro cell compression stimulator for evaluating real-time cellular responses to compression stimuli. The device was produced by a micro three-dimensional structure fabrication process using multiple exposures to the photoresist. The device consists of a pressure inlet port, cell inlet ports, a gasket, microchannels, cell culture chambers, and a diaphragm on the culture chamber for applying compressive pressure to cells. Compression stimuli applied to the cells can be controlled by regulating the expansion of the diaphragm via a pressure control. The device permits the observation of cellular responses to compressive pressure in real time because it is made of transparent materials and stimulates the cells without deforming the cell culture surface, when observed by optical microscopy. We demonstrated the validity of the fabrication process, evaluated the performance of the fabricated device, and compared the experimental results with the FEM structural analysis results. We found through operational testing that the diaphragm was deformed quickly by applying negative∕positive pressure and that the diaphragm displacement became larger with increasing applied pressure. These results indicate that this device can be used to control the intensity and the cell stimulus profile by regulating the applied pressure. In all cases, the cellular deformation during compression stimulus was successfully observed in real time using an optical microscope. The device is expected to facilitate the control of stem cell differentiation and the clarification of cellular mechanoreceptor mechanisms and signal transduction pathways.
Authors:
Y Nakashima; Y Yang; K Minami
Related Documents :
15477385 - Blood pressure and the cystic fibrosis gene: evidence for lower pressure rises with age...
17296875 - Corin i555(p568) allele is associated with enhanced cardiac hypertrophic response to in...
15375085 - The role of genetic and environmental factors in the association between birthweight an...
17339535 - Molecular genetics of experimental hypertension and the metabolic syndrome: from gene p...
2414585 - Increased cardiovascular responses to norepinephrine and calcium antagonists in essenti...
7296165 - Acute effects of propranolol on the circulation and on oxygen uptake in conscious rats.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Review of scientific instruments     Volume:  83     ISSN:  1089-7623     ISO Abbreviation:  Rev Sci Instrum     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-06-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0405571     Medline TA:  Rev Sci Instrum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  055004     Citation Subset:  IM    
Affiliation:
Graduate School of Science and Engineering, Yamaguchi University, 2-16-1 Tokiwadai, Ube, Yamaguchi 755-8611, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Tuning of nanogap size in high tensile stress silicon nitride thin films.
Next Document:  A pressure gauge based on gas density measurement from analysis of the thermal noise of an atomic fo...