| Development of membrane filter holder (MFH) method for recovery of heat-injured Escherichia coli O157:H7 and Salmonella typhimurium. | |
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MedLine Citation:
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PMID: 11849495 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: A method of recovering sublethally heat-injured bacteria was developed with specific apparatus (membrane filter holder; MFH) which was originally used for Iso-Grid Hydrophobic membrane. filter holder. METHODS AND RESULTS: The procedure used a non-selective agar underlayed with a selective medium with a MFH. A non-selective agar was poured on upper part (compartment A) of MFH, and then injured foodborne pathogens were inoculated on the non-selective medium. After 3-h repair incubation period, selective agar was added to the bottom of the chamber (compartment B) of the MFH and further incubated. By diffusing through the non-selective top agar, selective agents from the underlay medium impart selectivity to the system. CONCLUSIONS: Using the MFH method, recovery of heat-injured foodborne pathogens (Escherichia coli O157:H7 and Salmonella typhimurium) were not different (P > 0.05) from recoveries with non-selective media (TSA). However, the recoveries of foodborne pathogens on MFH were significantly higher (P < 0.05) than those of direct plating on selective medium such as SMAC (MacConkey Sorbitol Agar) or XLD (Xylose Lysine Desoxycholate). SIGNIFICANCE AND IMPACT OF THE STUDY: In conclusion, the MFH method is a simple and convenient method for recovery of heat-injured foodborne pathogens. |
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Authors:
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D H Kang |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Letters in applied microbiology Volume: 34 ISSN: 0266-8254 ISO Abbreviation: Lett. Appl. Microbiol. Publication Date: 2002 |
Date Detail:
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Created Date: 2002-02-18 Completed Date: 2002-03-19 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8510094 Medline TA: Lett Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 62-6 Citation Subset: IM |
Affiliation:
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Department of Food Science and Human Nutrition, Washington State University, Pullman, WA 99164-6376, USA. dhkang@wsu.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Culture Media Escherichia coli O157 / growth & development, isolation & purification* Filtration Hot Temperature Salmonella typhimurium / growth & development, isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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