Document Detail

Development of mammalian embryos exposed to mixed-size nanoparticles.
MedLine Citation:
PMID:  16440818     Owner:  NLM     Status:  MEDLINE    
Inhaled or ingested ultrafine nanoparticles and their effects on early pregnancy remain polemic. The objectives of the study were: (a) to determine the embryotoxic effects of nanoparticles at the 2-cell stage and (b) to localize the internalized nanoparticles in the blastocyst. Thawed mouse 2-cell embryos (no. = 128) were exposed to either mixed-size polystyrene-based nanoparticles (11 million/ml) or control G1.3 medium and assessed after 72 hours. Additionally, blastocysts (no. = 146) were exposed to nanoparticles and analyzed. The results showed that the nanoparticles did not inhibit 2-cell embryo development to the blastocyst stage (89.4 vs 96.8%; treated vs control). There were no differences in hatching (34.8 vs 43.5%), implantation (13.6 vs 24.2%) and degeneration (10.6 vs 3.2%). Delayed exposure to nanoparticles showed similar percent hatching (40.7 vs 47.3%) and implantation (17.6 vs 20.0%). Although nanoparticles were internalized, embryo development was not inhibited suggesting a lack of embryotoxicity. During hatching, the larger nanoparticles adhered to the extruding blastocyst, preferentially on trophoblasts, but interference was insignificant. Exposure to polystyrene-based nanoparticles at the concentration tested are not associated with embryonic loss.
S J Bosman; S P Nieto; W C Patton; J D Jacobson; J U Corselli; P J Chan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental obstetrics & gynecology     Volume:  32     ISSN:  0390-6663     ISO Abbreviation:  Clin Exp Obstet Gynecol     Publication Date:  2005  
Date Detail:
Created Date:  2006-01-30     Completed Date:  2006-03-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7802110     Medline TA:  Clin Exp Obstet Gynecol     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  222-4     Citation Subset:  IM    
Department of Physiology, Loma Linda University School of Medicine, Loma Linda, CA 92354, USA.
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MeSH Terms
Embryo Implantation
Embryo, Mammalian
Embryonic Development / physiology*
Nanostructures / toxicity*
Reg. No./Substance:

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