| Development of mammalian embryos exposed to mixed-size nanoparticles. | |
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MedLine Citation:
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PMID: 16440818 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inhaled or ingested ultrafine nanoparticles and their effects on early pregnancy remain polemic. The objectives of the study were: (a) to determine the embryotoxic effects of nanoparticles at the 2-cell stage and (b) to localize the internalized nanoparticles in the blastocyst. Thawed mouse 2-cell embryos (no. = 128) were exposed to either mixed-size polystyrene-based nanoparticles (11 million/ml) or control G1.3 medium and assessed after 72 hours. Additionally, blastocysts (no. = 146) were exposed to nanoparticles and analyzed. The results showed that the nanoparticles did not inhibit 2-cell embryo development to the blastocyst stage (89.4 vs 96.8%; treated vs control). There were no differences in hatching (34.8 vs 43.5%), implantation (13.6 vs 24.2%) and degeneration (10.6 vs 3.2%). Delayed exposure to nanoparticles showed similar percent hatching (40.7 vs 47.3%) and implantation (17.6 vs 20.0%). Although nanoparticles were internalized, embryo development was not inhibited suggesting a lack of embryotoxicity. During hatching, the larger nanoparticles adhered to the extruding blastocyst, preferentially on trophoblasts, but interference was insignificant. Exposure to polystyrene-based nanoparticles at the concentration tested are not associated with embryonic loss. |
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Authors:
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S J Bosman; S P Nieto; W C Patton; J D Jacobson; J U Corselli; P J Chan |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical and experimental obstetrics & gynecology Volume: 32 ISSN: 0390-6663 ISO Abbreviation: Clin Exp Obstet Gynecol Publication Date: 2005 |
Date Detail:
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Created Date: 2006-01-30 Completed Date: 2006-03-30 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7802110 Medline TA: Clin Exp Obstet Gynecol Country: Italy |
Other Details:
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Languages: eng Pagination: 222-4 Citation Subset: IM |
Affiliation:
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Department of Physiology, Loma Linda University School of Medicine, Loma Linda, CA 92354, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst* Embryo Implantation Embryo, Mammalian Embryonic Development / physiology* Mice Nanostructures / toxicity* Polystyrenes |
| Chemical | |
Reg. No./Substance:
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0/Polystyrenes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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