| Development of malaria parasites in mosquitoes fed with ookinetes suspended in defined media. | |
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MedLine Citation:
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PMID: 2406164 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Information concerning the specific nutritional requirements of malarial parasites developing in the mosquito host has been difficult to obtain, owing primarily to the complex nature of the blood meal that accompanies the parasites and the lack of success in culturing the complete invertebrate cycle of Plasmodium in vitro. The present report describes a blood-free system for infecting mosquitoes with ookinetes of Plasmodium berghei and for allowing the latter to develop into infective sporozoites. Ookinetes cultured in vitro were separated from blood proteins, suspended in defined medium, and fed to Anopheles stephensi mosquitoes through a membrane. The mosquitoes were then maintained on the same defined medium plus 5% sucrose. Infectivity of the parasites was demonstrated 17-19 days later by intracardial inoculation of the macerated mosquitoes into hamsters. This system makes it possible to evaluate nutritional factors that affect parasite development in the mosquito host under controlled conditions. |
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Authors:
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M Samish |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experientia Volume: 46 ISSN: 0014-4754 ISO Abbreviation: Experientia Publication Date: 1990 Feb |
Date Detail:
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Created Date: 1990-03-29 Completed Date: 1990-03-29 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0376547 Medline TA: Experientia Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 224-5 Citation Subset: IM |
Affiliation:
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Department of Entomology and Economic Zoology, Cook College, Rutgers University, New Brunswick, New Jersey 08903. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anopheles / parasitology* Culture Media Plasmodium berghei / growth & development* |
| Grant Support | |
ID/Acronym/Agency:
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AI-18345/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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