Document Detail

Development of macroglial cells in rat cerebellum. I. Use of antibodies to follow early in vivo development and migration of oligodendrocytes.
MedLine Citation:
PMID:  3047324     Owner:  NLM     Status:  MEDLINE    
The origin of oligodendrocytes and astrocytes in the CNS is still a focus of much experimentation and controversy. We have used antibodies against ganglioside GD3 and galactocerebroside (GC) to follow the origin and development of rat cerebellar oligodendrocytes both in vitro and in vivo. The immunofluorescent identification of GC+ cells in the rat neonatal cerebellum in vivo, revealed that cells initially GD3+/GC- appeared to make the transition via GD3+/GC+ cells to GD3-/GC+ oligodendrocytes. This sequence of events closely paralleled the maturation of cerebellar oligodendrocyte precursors found in serum-free dissociated culture. In contrast, whereas both GD3+ and glial fibrillary acidic protein-positive cells were seen in serum-containing dissociated culture and also in freshly dissociated suspensions of cerebellum at postnatal days 0 to 6, such cells could not be identified in situ. Putative GD3+/GC- oligodendrocyte precursor cells arose from the deeper regions of the cerebellum at birth, perhaps initially from the superior medullary velum adjacent to the fourth ventricle, and appeared to migrate into the developing folia just prior to myelination and the acquisition of GC.
R Curtis; J Cohen; J Fok-Seang; M R Hanley; N A Gregson; R Reynolds; G P Wilkin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurocytology     Volume:  17     ISSN:  0300-4864     ISO Abbreviation:  J. Neurocytol.     Publication Date:  1988 Feb 
Date Detail:
Created Date:  1988-10-24     Completed Date:  1988-10-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0364620     Medline TA:  J Neurocytol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  43-54     Citation Subset:  IM    
Biochemistry Department, Imperial College of Science and Technology, London, UK.
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MeSH Terms
Antibodies, Monoclonal / diagnostic use
Cell Count
Cell Movement
Cells, Cultured
Cerebellum / cytology,  growth & development*
Fluorescent Antibody Technique
Galactosylceramides / analysis
Gangliosides / analysis
Glial Fibrillary Acidic Protein / analysis
Neuroglia / physiology*
Oligodendroglia / physiology*
Specimen Handling
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Galactosylceramides; 0/Gangliosides; 0/Glial Fibrillary Acidic Protein; 62010-37-1/ganglioside, GD3

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