Document Detail


Development of lymphatic filarial parasite Wuchereria bancrofti (Spirurida: Onchocercidae) in mosquito species (Diptera: Culicidae) fed artificially on microfilaremic blood.
MedLine Citation:
PMID:  17162957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The efficiency of laboratory colonies of mosquitoes such as Anopheles stephensi Liston, Aedes aegypti (L.) Liverpool strain, Ae. aegypti wild type, Aedes albopictus (Skuse), Culex tritaeniorhynchus Giles, Culex sitiens Wiedemann, and Armigeres subalbatus Coquillett in supporting the development of Wuchereria bancrofti (Cobbold) (Spirurida: Onchocercidae) microfilariae to infective larvae was investigated. The mosquitoes were fed on heparinized microfilaremic human blood by using a membrane-feeding unit with Parafilm as membrane. The rate of infection, parasite development, and parasite burden were compared with that in the known vector mosquito Culex quinquefasciatus Say. Cx. quinquefasciatus showed the highest percentage of infection, followed by Ae. aegypti Liverpool strain and An. stephensi. The rate of development of the parasite was more or less similar in all the three species, and infective larvae were found on day 13. When the larvae were harvested on day 17, Cx. quinquefasciatus yielded the highest numbers, followed by Ae. aegypti Liverpool strain and An. stephensi. The percentage of infection was low, and the development was slow in Cx. tritaeniorhynchus compared with the other susceptible species. The parasite developed to second-stage larvae only by day 22 and to infective larvae by day 28. When 2-wk-old Cx. tritaeniorhynchus were fed on microfilaremic blood, they could develop the parasite to infective larvae by day 13 postfeeding. All other species of mosquitoes tested were found to be refractory to parasite development. It is shown that Cx. quinquefasciatus is the most suitable mosquito host for the production of infective larvae. However, Ae. aegypti Liverpool strain, which is commonly used for Brugia malayi filarial parasite, also can be used for generation of W. bancrofti infective larvae to circumvent the problem of maintaining two mosquito species.
Authors:
K P Paily; S L Hoti; K Balaraman
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of medical entomology     Volume:  43     ISSN:  0022-2585     ISO Abbreviation:  J. Med. Entomol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-12-13     Completed Date:  2007-01-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0375400     Medline TA:  J Med Entomol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1222-6     Citation Subset:  IM    
Affiliation:
Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, Pondicherry 605 006, India. kpaily@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Animal Nutritional Physiological Phenomena*
Animals
Blood
Culicidae / parasitology*,  physiology*
Host-Parasite Interactions
Laboratory Animal Science / methods
Species Specificity
Time Factors
Wuchereria bancrofti / growth & development*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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