Document Detail


Development of a long-term ovine model of cutaneous burn and smoke inhalation injury and the effects of early excision and skin autografting.
MedLine Citation:
PMID:  22459154     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MATERIALS AND METHODS: Eighteen female sheep were surgically prepared for this study under isoflurane anesthesia. The animals were divided into three groups: an Early Excision group (20% TBSA, third-degree cutaneous burn and 36 breaths of cotton smoke followed by early excision and skin autografting at 24h after injury, n=6), a Control group (20% TBSA, third-degree cutaneous burn and 36 breaths of cotton smoke without early excision, n=6) and a Sham group (no injury, no early excision, n=6). After induced injury, all sheep were placed on a ventilator and fluid-resuscitated with Lactated Ringers solution (4 mL/% TBS/kg). At 24h post-injury, early excision was carried out to fascia, and skin grafting with meshed autografts (20/1000 in., 1:4 ratio) was performed under isoflurane anesthesia. At 48 h post-injury, weaning from ventilator was begun if PaO(2)/FiO(2) was above 250 and sheep were monitored for 3 weeks.
RESULTS: At 96 h post-injury, all animals were weaned from ventilator. There are no significant differences in PaO(2)/FiO(2) between Early Excision and Control groups at any points. All animals were survived for 3 weeks without infectious complication in Early Excision and Sham groups, whereas two out of six animals in the Control group had abscess in lung. The percentage of the wound healed surviving area (mean ± SD) was 74.7 ± 7.8% on 17 days post-surgery in the Early Excision group. Lung wet-to-dry weight ratio (mean ± SD) was significantly increased in the Early Excision group vs. Sham group (p<0.05). The calculated net fluid balance significantly increased in the early excision compared to those seen in the Sham and Control groups. Plasma protein, oncotic pressure, hematocrit of % baseline, hemoglobin of % baseline, white blood cell and neutrophil were significantly decreased in the Early Excision group vs. Control group.
CONCLUSIONS: The early excision model closely resembles practice in a clinical setting and allows long-term observations of pulmonary function following burn and smoke inhalation injury. Further studies are warranted to assess lung tissue scarring and measuring collagen deposition, lung compliance and diffusion capacity.
Authors:
Yusuke Yamamoto; Perenlei Enkhbaatar; Hiroyuki Sakurai; Sebastian Rehberg; Sven Asmussen; Hiroshi Ito; Linda E Sousse; Robert A Cox; Donald J Deyo; Lillian D Traber; Maret G Traber; David N Herndon; Daniel L Traber
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-03-27
Journal Detail:
Title:  Burns : journal of the International Society for Burn Injuries     Volume:  38     ISSN:  1879-1409     ISO Abbreviation:  Burns     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-07-23     Completed Date:  2012-11-06     Revised Date:  2013-12-06    
Medline Journal Info:
Nlm Unique ID:  8913178     Medline TA:  Burns     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  908-16     Citation Subset:  IM    
Copyright Information:
Published by Elsevier Ltd.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Burns / surgery*
Chronic Disease
Disease Models, Animal*
Female
Hematocrit
Pulmonary Gas Exchange / physiology
Sheep
Skin Transplantation / methods*
Smoke Inhalation Injury / physiopathology*
Time Factors
Vascular Resistance / physiology
Grant Support
ID/Acronym/Agency:
GM66312-01/GM/NIGMS NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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