| Development of an on-line SPE-LC-ESI-MS method for urinary nucleosides: hyphenation of aprotic boronic acid chromatography with hydrophilic interaction LC-ESI-MS. | |
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MedLine Citation:
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PMID: 18198895 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The development of an on-line automated SPE-HPLC--ESI-MS method is described for targeted metabolomic analysis of urinary modified nucleoside levels. The setup comprises a boronate affinity column as a trapping device, a hydrophilic interaction chromatography (HILIC) separation and information-dependent MS detection modes. The system was optimized using standards and tested on biological samples, detecting a number of modified nucleosides. Other urinary biomarkers could also be analyzed by the system developed: for example, the urinary nucleobases were also available for analysis. A simultaneous creatinine-monitoring experiment was also demonstrated to be viable when utilizing the method, which is of benefit as creatinine is a urinary normalizing factor. |
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Authors:
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Robin Tuytten; Filip Lemière; Walter Van Dongen; Erwin Witters; Eddy L Esmans; Russell P Newton; Ed Dudley |
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Publication Detail:
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Type: Journal Article Date: 2008-01-17 |
Journal Detail:
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Title: Analytical chemistry Volume: 80 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-02-15 Completed Date: 2008-05-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1263-71 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Nucleoside Research and Mass Spectrometry Unit and Center for Proteomics and Mass Spectrometry, University of Antwerp, Antwerp, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Boronic Acids / chemistry Chromatography, High Pressure Liquid / methods* Creatinine / urine Humans Nucleosides / urine* Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization / methods* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Boronic Acids; 0/Nucleosides; 60-27-5/Creatinine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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