| Development of a lavage procedure to collect crop secretions from live chickens for studying crop immunity. | |
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MedLine Citation:
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PMID: 12593742 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The crop (ingluvies), an organ for food storage in most avian species, is located at the base of the oesophagus. Previous work in our laboratory showed that, following infection with Salmonella enteritidis, significant anti-S. enteritidis antibody levels could be found in the crops of these birds. Samples in these previous studies were obtained by flushing the interiors of crops excised from killed birds, which is both labour and animal intensive. A method was sought that allowed multiple sampling of the same birds over time. We found that lavage fluid could be administered directly into the crop down the oesophagus using a narrow-diameter plastic tubing attached to a syringe, and the fluid could then be aspirated back into the syringe. Antibody-containing crop secretions could be collected with minimal discomfort to the test animals. In a study where birds were challenged with S. enteritidis, immunoglobulin A anti-S. enteritidis titres 3 weeks post-challenge were similar in crop samples obtained by live lavage versus the flushing of crops removed from killed birds. Such a sampling procedure may provide researchers with a simple method to follow mucosal immunity in chickens following infection or vaccination regimens. |
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Authors:
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Peter S Holt; Lara E Vaughn; Richard K Gast; Henry D Stone |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Avian pathology : journal of the W.V.P.A Volume: 31 ISSN: 0307-9457 ISO Abbreviation: Avian Pathol. Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2003-02-20 Completed Date: 2003-09-12 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8210638 Medline TA: Avian Pathol Country: England |
Other Details:
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Languages: eng Pagination: 589-92 Citation Subset: IM |
Affiliation:
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USDA/ARS Southeast Poultry Research Laboratory, 934 College Station Road, Athens, GA 30605, USA. pholt@seprl.usda.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Bacterial / analysis Chickens / immunology* Crop, Avian / immunology*, secretion* Irrigation / methods*, veterinary* Salmonella enteritidis / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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