Document Detail


Development of a laboratory scale clean-in-place system to test the effectiveness of "natural" antimicrobials against dairy biofilms.
MedLine Citation:
PMID:  15270498     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A laboratory scale system, partially reproducing dairy plant conditions, was developed to quantify the effectiveness of chlorine and alternative sanitizers in reducing the number of viable bacteria attached to stainless steel surfaces. Stainless steel tubes fouled in a continuous flow reactor were exposed to a standard clean-in-place regime (water rinse, 1% sodium hydroxide at 70 degrees C for 10 min, water rinse, 0.8% nitric acid at 70 degrees C for 10 min, water rinse) followed by exposure to either chlorine (200 ppm) or combinations of nisin (500 ppm), lauricidin (100 ppm), and the lactoperoxidase system (LPS) (200 ppm) for 10 min or 2, 4, 8, 18, or 24 h. There was significant variation in the effectiveness of the alkaline-acid wash steps in reducing cell numbers (log reduction between 0 and 2). Following a 10-min treatment, none of the sanitizers significantly reduced the number of attached cells. Two hours of exposure to chlorine, nisin + the LPS, or lauricidin + the LPS achieved 2.8, 2.2, and 1.6 log reductions, respectively. Exposure times > 2 h did not further decrease the number of viable bacteria attached to the stainless steel. The effectiveness of combinations of nisin, lauricidin, and the LPS was similar to that of chlorine (P > 0.05), and these sanitizers could be used to decontaminate the surfaces of small-volume or critical hard-to-clean milk processing equipment.
Authors:
Muriel Dufour; Robin S Simmonds; Phil J Bremer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  67     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-07-23     Completed Date:  2004-09-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1438-43     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Otago, P.O. Box 56, Dunedin, New Zealand.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Bacterial Agents / pharmacology*
Bacteria / drug effects*,  growth & development
Cattle
Chlorine / pharmacology
Colony Count, Microbial
Dairying / methods
Drug Synergism
Equipment Contamination*
Female
Food Microbiology
Glycerides / pharmacology
Lactoperoxidase
Laurates / pharmacology
Milk / microbiology*
Monoglycerides
Nisin / pharmacology
Nitric Acid / pharmacology
Sodium Hydroxide / pharmacology
Stainless Steel
Time Factors
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Glycerides; 0/Laurates; 0/Monoglycerides; 12597-68-1/Stainless Steel; 1310-73-2/Sodium Hydroxide; 1414-45-5/Nisin; 27215-38-9/monolaurin; 7697-37-2/Nitric Acid; 7782-50-5/Chlorine; EC 1.11.1.-/Lactoperoxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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