Document Detail

Development of an ischemic tolerance model in a PC12 cell line.
MedLine Citation:
PMID:  15647748     Owner:  NLM     Status:  MEDLINE    
Although ischemic tolerance has been described in a variety of primary cell culture systems, no similar in vitro models have been reported with any cell line. A model of ischemic preconditioning in the rat pheochromocytoma PC12 cell line is described here. When compared to nonpreconditioned cells, preexposure of PC12 cells to 6 hours of oxygen and glucose deprivation (OGD) significantly increased cell viability after 15 hours of OGD 24 hours later. Flow cytometry analysis of cells labeled with specific markers for apoptosis, Annexin V, and Hoechst 33342, and of DNA content, revealed that apoptosis is involved in OGD-induced PC12 cell death and that preconditioning of the cells mainly counteracts the effect of apoptosis. Immunocytochemistry of caspase-3, a central executioner in the apoptotic process, further confirmed the activation of apoptotic pathways in OGD-induced PC12 cell death. This model may be useful to investigate the cellular mechanisms involved in neuronal transient tolerance following ischemia.
Joëlle A Hillion; Kenzo Takahashi; Dragan Maric; Christl Ruetzler; Jeffery L Barker; John M Hallenbeck
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     Volume:  25     ISSN:  0271-678X     ISO Abbreviation:  J. Cereb. Blood Flow Metab.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-28     Completed Date:  2005-03-03     Revised Date:  2012-03-07    
Medline Journal Info:
Nlm Unique ID:  8112566     Medline TA:  J Cereb Blood Flow Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  154-62     Citation Subset:  IM    
Stroke Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892-4128, USA.
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MeSH Terms
Annexin A5 / metabolism
Apoptosis / physiology
Benzimidazoles / metabolism
Caspase 3
Caspases / metabolism
Flow Cytometry
Ischemic Preconditioning / methods*
Models, Biological*
PC12 Cells / pathology*
Grant Support
Reg. No./Substance:
0/Annexin A5; 0/Benzimidazoles; 23491-52-3/HOE 33342; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

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