Document Detail


Development of an integrative DNA transformation system for the yeast Candida utilis.
MedLine Citation:
PMID:  9742706     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report here the development of an auxotrophic transformation system for the food yeast Candida utilis. To facilitate molecular studies in Candida utilis, we isolated auxotrophic strains for uracil biosynthesis by the combination of NTG-mutagenesis and 5-fluorotic acid (FOA) selection. The ura-mutation could be functionally complemented by the homologous URA3 gene. We used both, LiAc and electroporation methods to direct insertions at the ura3 locus through homologous recombination.
Authors:
L Rodríguez; F P Chávez; L Basabe; T Rivero; J M Delgado
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  165     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-10-27     Completed Date:  1998-10-27     Revised Date:  2009-07-24    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  335-40     Citation Subset:  IM    
Affiliation:
Bioindustry Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/Y12660
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MeSH Terms
Descriptor/Qualifier:
Blotting, Southern
Candida / genetics*,  growth & development
Electroporation
Fungal Proteins / genetics
Genetic Vectors
Lithium
Mutation
Plasmids / genetics
Transformation, Genetic*
Uracil / biosynthesis
Chemical
Reg. No./Substance:
0/Fungal Proteins; 66-22-8/Uracil; 7439-93-2/Lithium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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