Document Detail


Development and initial evaluation of PEG-stabilized bilayer disks as novel model membranes.
MedLine Citation:
PMID:  15617826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We show in this study that stable dispersions dominated by flat bilayer disks may be prepared from a carefully optimized mixture of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), cholesterol, and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-5000] [PEG-DSPE(5000)]. By varying the content of the latter component, the average diameter of the disks can be changed in the interval from about 15 to 60 nm. The disks show excellent long-term stability, and their size and structure remain unaltered in the temperature range between 25 and 37 degrees C. The utility of the disks as artificial model membranes was confirmed and compared to uni- and multilamellar liposomes in a series of drug partition studies. Data obtained by isothermal titration calorimetry and drug partition chromatography (also referred to as immobilized liposome chromatography) indicate that the bilayer disks may serve as an attractive and sometimes superior alternative to liposomes in studies aiming at the investigation of drug-membrane interactions. The disks may, in addition, hold great potential for structure/function studies of membrane-bound proteins. Furthermore, we suggest that the sterically stabilized bilayer disks may prove interesting as carriers for in vivo delivery of protein/peptide, as well as conventional amphiphilic and/or hydrophobic, drugs.
Authors:
Emma Johansson; Caroline Engvall; Maria Arfvidsson; Per Lundahl; Katarina Edwards
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biophysical chemistry     Volume:  113     ISSN:  0301-4622     ISO Abbreviation:  Biophys. Chem.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2004-12-24     Completed Date:  2005-08-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0403171     Medline TA:  Biophys Chem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  183-92     Citation Subset:  IM    
Affiliation:
Department of Physical Chemistry, Biomedical Center, Uppsala University, Box 579, SE-75123 Uppsala, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Cholesterol / chemistry
Hydrophobicity
Lipid Bilayers / chemistry*
Liposomes / chemistry*
Membranes
Models, Biological
Particle Size
Peptides / chemistry
Pharmaceutical Preparations / chemistry
Phosphatidylcholines / chemistry
Phosphatidylethanolamines / chemistry
Polyethylene Glycols / chemistry*
Proteins / chemistry
Surface Properties
Chemical
Reg. No./Substance:
0/Lipid Bilayers; 0/Liposomes; 0/Peptides; 0/Pharmaceutical Preparations; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Polyethylene Glycols; 0/Proteins; 4539-70-2/1,2-distearoyllecithin; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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