| Development of an in vivo bioassay method for allergy-preventive substances using hen-egg white lysozyme (HEL)-induced blood flow decrease. | |
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MedLine Citation:
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PMID: 16079499 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We discovered a phenomenon in which the blood flow in vein microcirculation markedly decreases in response to hen-egg white lysozyme (HEL)-sensitization without any change in blood pressure. Using this blood flow decrease as a guide, we developed an in vivo assay method to search for substances, which can prevent allergies. Antagonists of histamine, serotonin and platelet activating factor (PAF) did not affect the blood flow decrease in response to HEL-sensitization. On the other hand, cyclooxygenase (COX)-1, COX-2, thromboxane (TX) A(2), endothelin-1 (ET-1), prostacyclin (PGI(2)) and granulocytic elastase (GE) as well as nitric oxide (NO) from inducible NO synthase (iNOS) were involved in the blood flow decrease. Thus, these substances might injure vascular endothelial cells, and cause a decrease in blood flow in vein microcirculation. Our method can be used to search for preventive agents against allergies involving NO, COX-1, 2 and PGI(2). This is the first report to applying to an assay method the specific blood flow decrease to occur in the promotion stage of allergy. |
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Authors:
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Kyoko Ishiguro; Hisae Oku; Yoshimi Ueda; Emiko Iwaoka; Masaru Kunitomo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biological & pharmaceutical bulletin Volume: 28 ISSN: 0918-6158 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-08-04 Completed Date: 2005-11-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
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Languages: eng Pagination: 1490-5 Citation Subset: IM |
Affiliation:
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School of Pharmaceutical Sciences, Mukogawa Women's University, Koshien Kyuban-cho, Nishinomiya, Hyogo 663-8179, Japan. ishiguro@mwu.mukogawa-u.ac.jp |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Allergic Agents / pharmacology* Biological Assay* Egg White Mice Muramidase / pharmacology* Nitric Oxide / biosynthesis Regional Blood Flow / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Allergic Agents; 10102-43-9/Nitric Oxide; EC 3.2.1.17/Muramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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