Document Detail

Development and in vitro validation of anti-mesothelin biobodies that prevent CA125/Mesothelin-dependent cell attachment.
MedLine Citation:
PMID:  17560019     Owner:  NLM     Status:  MEDLINE    
Preventing peritoneal implantation of carcinoma cells could prolong ovarian cancer patient remission and survival. Peritoneal cells constitutively express mesothelin, a ligand for CA125 that is expressed by tumor cells. Thus blocking CA125/mesothelin-dependent cell attachment may prevent or delay peritoneal metastatic recurrence. We developed a high-throughput screening system for reagents able to block CA125/mesothelin-dependent cell attachment with a sensitive quantitative readout. Using a novel yeast-expression system to produce secreted, in vivo biotinylated proteins and biobodies (Bbs), we generated anti-mesothelin Bbs. Anti-mesothelin Bbs derived from high affinity yeast-display scFv detected both membrane-bound and soluble mesothelins and inhibited CA125/mesothelin-dependent cell attachment.
Lindsay Bergan; Jennifer A Gross; Barry Nevin; Nicole Urban; Nathalie Scholler
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-06-07
Journal Detail:
Title:  Cancer letters     Volume:  255     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-08-27     Completed Date:  2007-11-09     Revised Date:  2011-10-26    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  263-74     Citation Subset:  IM    
Molecular Diagnositics Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
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MeSH Terms
Amino Acid Sequence
Antibodies, Monoclonal / biosynthesis*,  genetics,  pharmacology*
Biological Assay
CA-125 Antigen / drug effects*
Cell Adhesion / drug effects*
Drug Screening Assays, Antitumor / methods
GPI-Linked Proteins
Immunoglobulin Variable Region / biosynthesis,  genetics,  pharmacology
Membrane Glycoproteins / antagonists & inhibitors*
Molecular Sequence Data
Peritoneal Neoplasms / prevention & control*,  secondary
Recombinant Proteins / biosynthesis,  genetics,  pharmacology
Saccharomyces cerevisiae / genetics,  metabolism
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/CA-125 Antigen; 0/GPI-Linked Proteins; 0/Immunoglobulin Variable Region; 0/Membrane Glycoproteins; 0/Recombinant Proteins; 0/mesothelin

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