Document Detail

Development of an in vitro psoriatic skin model by tissue engineering.
MedLine Citation:
PMID:  18783923     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Psoriasis is a chronic skin disease characterized by a thickening and disorganization of the skin's protective barrier. OBJECTIVES: This study aims to develop and characterize a novel in vitro psoriatic human skin model produced by tissue engineering. METHODS: The self-assembly method, a tissue engineering approach based on the capacity of mesenchymal cells, such as fibroblasts, to create their own extracellular matrix in vitro, was used to create our substitutes. Manipulatable sheets of fibroblasts were superimposed creating a new dermis upon which keratinocytes are seeded, leading to a complete bilayered skin substitute. The characterization of the psoriatic substitutes was performed by macroscopic, histological and immunohistochemical analyses and contrasted to those constructed from healthy cells. RESULTS: Macroscopically, the psoriatic substitutes were more white and thicker than the healthy substitutes. The histological analysis of psoriatic substitutes stained with Masson's trichrome revealed a characteristic thickening of the epidermal layer seen in psoriatic skin in vivo. Immunohistochemical analysis of the psoriatic substitutes showed, among other things, an overexpression of involucrin and an underexpression of filaggrin and loricrin. CONCLUSION: These data suggest that the macroscopic, histological and immunohistochemical characteristics of psoriasis are partially retained in the substitutes, thus providing a good model to investigate the mechanisms of abnormal keratinocyte growth and to study cell-cell interactions.
Jessica Jean; Marc Lapointe; Jacques Soucy; Roxane Pouliot
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-09
Journal Detail:
Title:  Journal of dermatological science     Volume:  53     ISSN:  0923-1811     ISO Abbreviation:  J. Dermatol. Sci.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-22     Completed Date:  2009-03-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9011485     Medline TA:  J Dermatol Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  19-25     Citation Subset:  IM    
Laboratoire d'Organogénèse Expérimentale, Hôpital du Saint-Sacrement du CHA, Québec, Canada.
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MeSH Terms
Cell Communication
Cell Proliferation
Extracellular Matrix / metabolism,  pathology
Fibroblasts / metabolism,  pathology
Intermediate Filament Proteins / metabolism
Keratinocytes / metabolism,  pathology
Membrane Proteins / metabolism
Middle Aged
Models, Biological*
Protein Precursors / metabolism
Psoriasis / metabolism,  pathology*
Skin / metabolism,  pathology*
Tissue Engineering / methods*
Reg. No./Substance:
0/Intermediate Filament Proteins; 0/Membrane Proteins; 0/Protein Precursors; 0/filaggrin; 0/loricrin; 60108-77-2/involucrin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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