Document Detail


Development of an in vitro infectivity assay for the C-type bovine leukemia virus.
MedLine Citation:
PMID:  175950     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of the bovine C-type leukemia virus to induce syncytia formation in monolayer cell cultures has been used to develop a specific and simple infectivity assay for the virus. Using bovine embryonic spleen cells or human diploid embryonic lung cells as indicator cells, the results of the assay can be evaluated in 4 to 6 or 6 to 8 days, respectively. Pretreatment of the indicator cells with DEAE-dextran greatly increases the sensitivity of the assay. The assay is quantitative and can be applied as a direct method for the identification of bovine C-type leukemia virus-infected animals; it also provides a simple, and sensitive procedure for the detection and titration of virus-neutralizing antibodies.
Authors:
J F Ferrer; C A Diglio
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  36     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1976 Mar 
Date Detail:
Created Date:  1976-06-02     Completed Date:  1976-06-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1068-73     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Viral / analysis
Cells, Cultured
Cytopathogenic Effect, Viral*
Leukemia Virus, Bovine / immunology,  isolation & purification*
Neutralization Tests
Retroviridae / isolation & purification*
Chemical
Reg. No./Substance:
0/Antibodies, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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