Document Detail


Development in electrofused conjugants of tetrahymena thermophila.
MedLine Citation:
PMID:  11411835     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Electric shock can create parabiotic fusions of living Tetrahymena cells. In this study, cells were mated and successful pairs were electrofused with either vegetatively growing cells or other mating pairs. In particular, we electrofused pairs from normal [diploid x diploid] matings with vegetatively dividing cells in G- or M-phase of the cell cycle. We also fused [diploid x diploid] conjugants with mating pairs involving an aneuploid partner [diploid x "star"], which typically undergo an abortive conjugal pathway termed genomic exclusion. Using such parabiotic fusions we identified and characterized two developmentally critical landmarks: 1) the "abort" signal, which is initiated in pairs with nuclear defects (this first becomes evident soon after the completion of Meiosis I or the beginning of Meiosis II); and 2) the "terminal commitment point", a developmental stage in normal [diploid x diploid] pairs after which conjugation no longer responds to a parabiotically transmitted abort signal (this correlates with the onset of the second postzygotic nuclear division). Finally we demonstrate that a conjugal-arrest-activity varies with the vegetative cell cycle, reaching its highest level of activity during M-phase and dropping just after cytokinesis.
Authors:
E S Cole; M A Virtue; K R Stuart
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of eukaryotic microbiology     Volume:  48     ISSN:  1066-5234     ISO Abbreviation:  J. Eukaryot. Microbiol.     Publication Date:    2001 May-Jun
Date Detail:
Created Date:  2001-06-19     Completed Date:  2001-11-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9306405     Medline TA:  J Eukaryot Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  266-79     Citation Subset:  IM    
Affiliation:
Biology Department, St. Olaf College, Northfield, Minnesota 55057, USA. Colee@stolaf.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fusion / methods
Diploidy
Electric Stimulation
Fluoresceins / chemistry
Fluorescent Dyes / chemistry
Indoles / chemistry
Microscopy, Fluorescence
Tetrahymena / cytology,  genetics,  growth & development*
Chemical
Reg. No./Substance:
0/Fluoresceins; 0/Fluorescent Dyes; 0/Indoles; 47165-04-8/DAPI; 79955-27-4/5-carboxyfluorescein diacetate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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