Document Detail


Development of an implantable D-serine biosensor for in vivo monitoring using mammalian D-amino acid oxidase on a poly (o-phenylenediamine) and Nafion-modified platinum-iridium disk electrode.
MedLine Citation:
PMID:  19945264     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
D-serine has been implicated as a brain messenger, promoting not only neuronal signalling but also synaptic plasticity. Thus, a sensitive tool for D-serine monitoring in brain is required to understand the mechanisms of D-serine release from glia cells. A biosensor for direct fixed potential amperometric monitoring of D-serine incorporating mammalian D-amino acid oxidase (DAAO) immobilized on a Nafion coated poly-ortho-phenylenediamine (PPD) modified Pt-Ir disk electrode was therefore developed. The combined layers of PPD and Nafion enhanced the enzyme activity and biosensor efficiency by approximately 2-fold compared with each individual layer. A steady state response time (t(90%)) of 0.7+/-0.1s (n=8) and limit of detection 20+/-1 nM (n=8) were obtained. Cylindrical geometry showed lower sensitivity compared to disk geometry (61+/-7 microA cm(-2) mM(-1), (n=4), R(2)=0.999). Interference by ascorbic acid (AA), the main interference species in the central nervous system and other neurochemical electroactive molecules was negligible. Implantation of the electrode and microinjection of D-serine into rat brain striatal extracellular fluid demonstrated that the electrode was capable of detecting D-serine in brain tissue in vivo.
Authors:
Zainiharyati M Zain; Robert D O'Neill; John P Lowry; Kenneth W Pierce; Mark Tricklebank; Aidiahmad Dewa; Sulaiman Ab Ghani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-06
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  25     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-03     Completed Date:  2010-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  1454-9     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier B.V. All rights reserved.
Affiliation:
Pusat Pengajian Sains Kimia, Universiti Sains Malaysia, 11800 USM Pulau Pinang, Malaysia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Conductometry / instrumentation*
Corpus Striatum / metabolism*
D-Amino-Acid Oxidase / chemistry*
Electrodes, Implanted*
Enzymes, Immobilized / chemistry
Fluorocarbon Polymers / chemistry
Humans
Iridium / chemistry
Male
Phenylenediamines / chemistry
Platinum / chemistry
Prostheses and Implants*
Rats
Rats, Sprague-Dawley
Serine / analysis,  metabolism*
Chemical
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Fluorocarbon Polymers; 0/Phenylenediamines; 0/poly(o-phenylenediamine); 39464-59-0/perfluorosulfonic acid; 56-45-1/Serine; 7439-88-5/Iridium; 7440-06-4/Platinum; EC 1.4.3.-/Dao1 protein, mouse; EC 1.4.3.3/D-Amino-Acid Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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