| Development of an implantable D-serine biosensor for in vivo monitoring using mammalian D-amino acid oxidase on a poly (o-phenylenediamine) and Nafion-modified platinum-iridium disk electrode. | |
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MedLine Citation:
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PMID: 19945264 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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D-serine has been implicated as a brain messenger, promoting not only neuronal signalling but also synaptic plasticity. Thus, a sensitive tool for D-serine monitoring in brain is required to understand the mechanisms of D-serine release from glia cells. A biosensor for direct fixed potential amperometric monitoring of D-serine incorporating mammalian D-amino acid oxidase (DAAO) immobilized on a Nafion coated poly-ortho-phenylenediamine (PPD) modified Pt-Ir disk electrode was therefore developed. The combined layers of PPD and Nafion enhanced the enzyme activity and biosensor efficiency by approximately 2-fold compared with each individual layer. A steady state response time (t(90%)) of 0.7+/-0.1s (n=8) and limit of detection 20+/-1 nM (n=8) were obtained. Cylindrical geometry showed lower sensitivity compared to disk geometry (61+/-7 microA cm(-2) mM(-1), (n=4), R(2)=0.999). Interference by ascorbic acid (AA), the main interference species in the central nervous system and other neurochemical electroactive molecules was negligible. Implantation of the electrode and microinjection of D-serine into rat brain striatal extracellular fluid demonstrated that the electrode was capable of detecting D-serine in brain tissue in vivo. |
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Authors:
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Zainiharyati M Zain; Robert D O'Neill; John P Lowry; Kenneth W Pierce; Mark Tricklebank; Aidiahmad Dewa; Sulaiman Ab Ghani |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-06 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 25 ISSN: 1873-4235 ISO Abbreviation: Biosens Bioelectron Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-03 Completed Date: 2010-04-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
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Languages: eng Pagination: 1454-9 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier B.V. All rights reserved. |
Affiliation:
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Pusat Pengajian Sains Kimia, Universiti Sains Malaysia, 11800 USM Pulau Pinang, Malaysia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Conductometry / instrumentation* Corpus Striatum / metabolism* D-Amino-Acid Oxidase / chemistry* Electrodes, Implanted* Enzymes, Immobilized / chemistry Fluorocarbon Polymers / chemistry Humans Iridium / chemistry Male Phenylenediamines / chemistry Platinum / chemistry Prostheses and Implants* Rats Rats, Sprague-Dawley Serine / analysis, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Enzymes, Immobilized; 0/Fluorocarbon Polymers; 0/Phenylenediamines; 0/poly(o-phenylenediamine); 39464-59-0/perfluorosulfonic acid; 56-45-1/Serine; 7439-88-5/Iridium; 7440-06-4/Platinum; EC 1.4.3.-/Dao1 protein, mouse; EC 1.4.3.3/D-Amino-Acid Oxidase |
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