Document Detail


Development of an impedimetric immunosensor for the determination of 3-amino-2-oxazolidone residue in food samples.
MedLine Citation:
PMID:  21995918     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The use of furazolidone in food animals has been banned in European Union (EU) because of its carcinogenicity and mutagenicity on human health, but its continued misuse is widespread. Therefore, there is an urgent need for a simple, reliable, and rapid method for the detection of its marker residue, 3-amino-2-oxazolidinone (AOZ), in food products. In this regard, a sensitive and reliable electrochemical method was presented to detect AOZ based on a novel label-free electrochemical impedimetric immunosensor to address this need. The immobilization of monoclonal antibody against AOZ (denoted as AOZ-McAb) on the gold electrode was carried out through a stable acyl amino ester intermediate generated by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydrosuccinimide (NHS), which could condense antibodies on the self-assembled monolayer (SAM). The detection of AOZ was performed by measuring the relative change in charge transfer resistance before and after AOZ and AOZ-McAb immunoreaction by electrochemical impedance spectroscopy (EIS). Under the optimized conditions, the relative change in charge transfer resistance was proportional to the logarithmic value of AOZ concentrations in the range of 20.0 to 1.0×10(4)ngmL(-1) (r=0.9987). Moreover, the proposed immunosensor has a high selectivity to AOZ alone with no significant response to the metabolites of other nitrofuran antibiotics, such as 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), semicarbazide (SEM), and 1-aminohydantoin hydrochloride (AHD). This protocol has been applied to detect AOZ in food samples with satisfactory results.
Authors:
Gongjun Yang; Wenjie Jin; Liping Wu; Qianqian Wang; Hongxia Shao; Aijian Qin; Bing Yu; Dongming Li; Baoliang Cai
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Publication Detail:
Type:  Journal Article     Date:  2011-09-01
Journal Detail:
Title:  Analytica chimica acta     Volume:  706     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-10-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  120-7     Citation Subset:  IM    
Copyright Information:
Crown Copyright © 2011. Published by Elsevier B.V. All rights reserved.
Affiliation:
School of Pharmacy, China Pharmaceutical University, Nanjing 210009, PR China.
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