| Development of a homologous transformation system for Aspergillus aculeatus based on the sC gene encoding ATP-sulfurylase. | |
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MedLine Citation:
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PMID: 19420695 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A homologous transformation system was developed using the endogenous ATP-sulfurylase gene, AasC, as a selectable marker in Aspergillus aculeatus. Spontaneous mutation was proved to be beneficial in isolating AasC-deficient mutants. Molecular analysis of sC(+) transformants revealed that the frequency of single copy integration at ATP-sulfurylase locus was more than 40%. |
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Authors:
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Hiromi Adachi; Shuji Tani; Shin Kanamasa; Jun-ichi Sumitani; Takashi Kawaguchi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 73 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-05-25 Completed Date: 2009-08-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 1197-9 Citation Subset: IM |
Affiliation:
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Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aspergillus
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genetics* Genetic Engineering / methods* Genome Mutation Sulfate Adenylyltransferase / genetics* Transformation, Genetic* |
| Chemical | |
Reg. No./Substance:
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EC 2.7.7.4/Sulfate Adenylyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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